biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 50:667-674, 2006 | DOI: 10.1007/s10535-006-0104-3

Protection of apple against fire blight induced by an hrpL mutant of Erwinia amylovora

M. Faize1, M. N. Brisset1, C. Perino2, B. Vian2, M. A. Barny2, J. P. Paulin1, M. Tharaud1,*
1 Unité Mixte de Recherche Pathologie Végétale 077, INRA, INH, Université d'Angers, Beaucouzé Cedex, France
2 Unité Mixte de Recherche 217, INRA, INA-PG, Université Paris VI, Laboratoire de Pathologie Végétale, Paris Cedex 05, France

A regulatory hrpL non-virulent mutant of Erwinia amylovora is effective in controlling fire blight disease when inoculated on apple seedlings simultaneously with the pathogenic parental strain. Mechanisms involved in this protective effect were investigated. The use of two marker genes, uidA and lacZ, expressed in the hrpL mutant and the pathogenic strain, respectively, allowed to localize simultaneously the two inoculated strains in plant tissue. An anti-β-glucuronidase antibody was also used to detect the hrpL mutant. Both techniques indicated that the two strains localized mainly in separate areas of the leaf tissue. In addition, leaves infiltrated with the hrpL mutant exhibited a significant increase in peroxidase activity in contrast to a hrp secretion mutant known to be less effective in the protection. It is suggested that protection obtained with the hrpL mutant relies on the physical separation between the mutant and the parental strain after co-inoculation and the rapid and sustained activation of plant defense mechanisms in reactive tissue, i.e. not invaded by the virulent strain.

Keywords: apple defense response; histochemical assays; Malus × domestica
Subjects: defence response; Erwinia amylovora; fire blight; β-galactosidase; β-glucuronidase; Malus domestica; mutagenesis, mutants; peroxidase

Received: March 10, 2004; Accepted: September 12, 2005; Published: December 1, 2006  Show citation

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Faize, M., Brisset, M.N., Perino, C., Vian, B., Barny, M.A., Paulin, J.P., & Tharaud, M. (2006). Protection of apple against fire blight induced by an hrpL mutant of Erwinia amylovora. Biologia plantarum, 50(4), 667-674. doi: 10.1007/s10535-006-0104-3
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