biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:693-696, 2006 | DOI: 10.1007/s10535-006-0108-z

In vitro regeneration of Trifolium glomeratum

P. Kaushal1,*, A. Tiwari1, A. K. Roy1, D. R. Malaviya1, B. Kumar1
1 Crop Improvement Division, Indian Grassland and Fodder Research Institute, Jhansi, India

In vitro regeneration of Trifolium glomeratum, a leguminous forage species, was attempted through leaf, petiole, cotyledon, hypocotyl, collar and root explants and two media combinations. Root and collar explants showed no callus induction. Medium with 0.05 mg dm-3 α-naphthaleneacetic acid (NAA) and 0.10 mg dm-3 N6-benzyladenine (BA) was more effective for hypocotyl explant whereas cotyledon and petiole explant were more responsive to 5.0 mg dm-3 NAA and 1.0 mg dm-3 BA. Friable, green calli obtained from petiole explant on this medium showed organogenetic potential. Modified root-inducing medium having 0.21 mg dm-3 indole-3-acetic acid and 2.5 % sucrose was successful for root induction and plantlets were successfully transferred to field after hardening and Rhizobium inoculation.

Keywords: auxins; clovers; cytokinins; tissue culture
Subjects: auxins; cotyledon; cytokinins; hypocotyl; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; Rhizobium inoculation; root, rooting; Trifolium glomeratum

Received: May 5, 2004; Accepted: June 27, 2005; Published: December 1, 2006  Show citation

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Kaushal, P., Tiwari, A., Roy, A.K., Malaviya, D.R., & Kumar, B. (2006). In vitro regeneration of Trifolium glomeratum. Biologia plantarum, 50(4), 693-696. doi: 10.1007/s10535-006-0108-z
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