biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:705-708, 2006 | DOI: 10.1007/s10535-006-0111-4

Gender-specific responses of Piper betle L. to low temperature stress: changes in chlorophyllase activity

N. Kumar1,*, S. Gupta1, A. N. Tripathi1
1 Betel Vine Biotechnology Laboratory, NBRI, Lucknow, India

Gender based differences in response to low temperature stress in leaf chlorophyll (Chl), and carotenoids (Car) contents and chlorophyllase (Chlase) activity were monitored in male (Kapoori Vellaikodi and Madras Pan Kapoori) and female (Bangla Mahoba, Desi Bangla and Kaker) betel vine landraces. Although female plants contained nearly two fold more Chl than male counterparts, the low temperature induced Chl loss was comparable, however, male plants showed higher Chl a/b ratio than females. Chlase activity increased due to cold stress in all the landraces. Male plants always showed higher activities of Chlase, which may be one of the reasons for the rather low Chl contents in male plants.

Keywords: betel vine; chilling; chlorophyll; dioecy; shade plant
Subjects: betel vine; carotenoids; chlorophyll a,b; chlorophyllase; dioecy; gender-based differences; (photosynthetic) photon flux density; photosystem 2; shade plant; temperature, low, stress

Received: September 23, 2004; Accepted: August 19, 2005; Published: December 1, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kumar, N., Gupta, S., & Tripathi, A.N. (2006). Gender-specific responses of Piper betle L. to low temperature stress: changes in chlorophyllase activity. Biologia plantarum, 50(4), 705-708. doi: 10.1007/s10535-006-0111-4
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Dawson, T.E., Bliss, L.C.: Patterns of water use and tissue water relations in the dioecious shrub, Salix artica: the physiological basis for habitat partitioning between sexes.-Oecologia 79: 332-343, 1989. Go to original source...
    2. Dawson, T.E., Geber, M.A.: Dimorphism in physiology and morphology.-In: Geber, M.A., Dawson, T.E., Delph, L.F. (ed).: Gender and Sexual Dimorphism in Flowering Plants. Pp. 175-216. Springer-Verlag, Berlin-Heidelberg 1999. Go to original source...
    3. Drążkiewicz, M.: Chlorophyllase: occurrence, function, mechanism of action, effects of external and internal factors.-Photosynthetica 30: 321-331, 1994.
    4. Drążkiewicz, M., Krupa, Z.: The participation of chlorophyllase in chlorophyll metabolism.-Acta Soc. Bot. Pol. 60: 139-154, 1991. Go to original source...
    5. Feierabend, J.: Capacity for chlorophyll synthesis in heat-bleached 70S ribosome-deficient rye leaves.-Planta 135: 83-88, 1977. Go to original source...
    6. Fernandez-Lopez, J.A., Almela, L., Almansa, M.S., Lopez-Roca, J.M.: Partial purification and properties of chlorophyllase from chlorotic Citrus limon leaves.-Phytochemistry 31: 447-449, 1992. Go to original source...
    7. Folly, P., Engel, N.: Chlorophyll b to chlorophyll a conversion precedes chlorophyll degradation in Hordeum vulgare L.-J. biol. Chem. 274: 21811-21816, 1999. Go to original source...
    8. Hendry, G.A.F., Stobart, A.K.: Chlorophyll turnover in greening barley.-Photochemistry 25: 2735-2737, 1986.
    9. Johnson-Flanagan, A.M., McLachlan, G.: The role of chlorophyllase in degreening canola (Brassica napus) seeds and its activation by sublethal freezing.-Physiol. Plant. 80: 460-466, 1990. Go to original source...
    10. Khalyfa, A., Kermasha, S., Marsot, P., Goetghebeur, M.: Purification and characterization of chlorophyllase from alga Phaeodactylum tricornutum by preparative native electrophoresis.-Appl. Biochem. Biotechnol. 53: 11-27, 1995. Go to original source...
    11. Kumar, N.: Betel vine (Piper betle L.) cultivation: a unique case of plant establishment under anthropogenically regulated microclimatic conditions.-Indian J. Hist. Sci. 33: 19-32, 1999.
    12. Lahiri, A.N., Singh, S.: Effects of hypothermia on the nitrogen metabolism of Pennisetum typhoides.-Proc. nat. Acad. Sci. India B 35: 131-138, 1969.
    13. Lichtenthaler, H.K.: Chlorophyll and carotenoids; pigments of photosynthetic biomembranes.-Methods Enzymol. 148: 352-382, 1987. Go to original source...
    14. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    15. Marshall, J.D., Dawson, T.E., Ehleringer, J.R.: Gender related differences in gas exhange are not related to host quality in the xylem-tapping mistletoe, Phoradendron juniperinum (Viscaceae).-Amer. J. Bot. 80: 641-645, 1993. Go to original source...
    16. Matile, P., Duggelin, T., Schellenberg, M., Rentsch, D., Bortlik, K., Peisker, C., Thomas, H.: How and why is chlorophyll broken down in senescent leaves?-Plant Physiol. Biochem. 27: 595-604, 1989.
    17. Matile, P., Hortensteiner, S., Thomas, H.: Chlorophyll degradation.-Annu. Rev. Plant Physiol. Plant. mol. Biol. 50; 67-95, 1999. Go to original source...
    18. Minguez-Mosquera, M.I., Gallardo-Guerrero, L: Role of chlorophyllase in chlorophyll metabolism in olives cv. Gordal.-Phytochemistry 41: 691-697, 1996. Go to original source...
    19. Nishiyama, Y., Kitamura, M., Tamura, S., Watanabe, T.: Purification and substrate specificity of chlorophyllase from Chlorella regularis.-Chem. Lett. 1: 69-72, 1994. Go to original source...
    20. Renner, S.S., Ricklefs, R.E.: Dioecy and its correlates in the flowering plants.-Amer. J. Bot. 82: 596-606, 1995. Go to original source...
    21. Rise, M., Goldschmidt, E.E.: Occurrence of chlorophyllides in developing light grown leaves of several species.-Plant Sci. 71: 147-151, 1990. Go to original source...
    22. Sheumann, V., Schoch, S., Rudiger, W.: Chlorophyll b reduction during senescence of barley seedlings.-Planta 209: 364-370, 1999. Go to original source...
    23. Shioi, Y., Tatsumi, Y., Shimokawa, K.: Enzymatic degradation of chlorophyll in Chenopodium album.-Plant Cell Physiol. 32: 87-93, 1991.
    24. Stobart, A.K., Hendry, G.A.F.: Chlorophyll turnover in greening wheat leaves-Photochemistry 23: 27-30, 1984. Go to original source...
    25. Stoll, A.: Über Chlorophyllase und die Chlorophyllide.-PhD. Thesis. ETH, Zürich 1912.
    26. Tanaka, K., Kakuno, T., Yamashita, J., Horio, T.: Purification and properties of chlorophyllase from greened rye seedlings.-J. Biochem. 92: 1763-1773, 1982. Go to original source...
    27. Tewari, A.K., Tripathi, B.C.: Acclimation of biosynthetic reactions to temperature stress in cucumber (Cucumis sativus L.).-Planta 208: 431-437, 1999. Go to original source...
    28. Todorov, D.T., Karanov, N.E., Smith, A.R., Hall, M.A.: Chlorophyllase activity and chlorophyll content in wild type and eti 5 mutant of Arabidopsis thaliana subjected to low and high temperatures.-Biol. Plant. 46: 633-636, 2003. Go to original source...
    29. Trebitsh, T., Goldschmidt, E.E., Riov, J.: Ethylene induces de novo synthesis of chlorophyllase, a chlorophyll degrading enzyme, in citrus fruit peel.-Proc. nat. Acad. Sci. USA 90: 9441-9445, 1993. Go to original source...
    30. Tripathi, R., Khare, G., Kumar, N.: Effect of low temperature stress on Piper betle L. types, Bangla and Desavari.-J. Spices Aromatic Crops 9: 141-143, 2000.
    31. Tsuchiya, T., Ohta, H., Masuda, T., Mikami, B., Kita, N., Shioi, Y., Takamiya, K.-I.: Purification and characterization of two isozymes of chlorophyllase from mature leaves of Chenopodium album.-Plant Cell Physiol. 38: 1026-1031, 1997. Go to original source...
    32. Tsuchiya, T., Ohta, H., Okawa, K., Iwamatsu, A., Shiomada, H., Masuda, T., Takamiya, K.-I.: Cloning of chlorophyllase gene the key enzyme in chlorophyll degradation: finding of a lipase motif and induction by methyl jasmonate.-Proc. nat. Acad. Sci. USA 96: 15362-15367, 1999. Go to original source...
    33. Tyagi, R.K., Prakash, S.: Genotype-and sex-specific protocols for in vitro micropropagation and medium-term conservation of jojoba.-Biol. Plant. 48: 19-23, 2004. Go to original source...
    34. Van Hasselt, P.R., Strikwerda, J.T.: Pigment degradation in discs of the thermophilic Cucumis sativus as affected by light, temperature, sugar application and inhibitors.-Plant Physiol. 37: 253-257, 1976. Go to original source...
    35. Wang, X., Curtis, P.S.: Gender-specific responses of Populus tremuloides to atmospheric CO2 enrichment.-New Phytol. 150: 675-684, 2001. Go to original source...

    Return to the content