Biologia plantarum 50:722-724, 2006 | DOI: 10.1007/s10535-006-0115-0
Effect of alginate matrix composition on regrowth of in vitro-derived encapsulated apical microcuttings of hybrid aspen
- 1 Tissue Culture Lab, Forest Research Institute, Sofia, Bulgaria
- 2 Department of Environment and Agrobiotechnology, CRP Gabriel Lippmann, Belvaux, Luxembourg
Effect of alginate matrix composition on regrowth performance of encapsulated microcuttings of hybrid aspen (Populus tremula L. × P. tremuloides Mincx.) was studied. Both high regrowth frequency and viability of explants were registered in all encapsulation mixtures tested. Some ingredients of the matrix (nutrient medium salts, sugars, growth regulators) significantly affected the initial development of the microcuttings. Sucrose appeared to play an important role in the starting stage of the regrowth event.
Keywords: encapsulation; propagules; Populus sp
Subjects: aspen; auxins; cytokinins; encapsulated microcuttings; in vitro culture, agar, alginate; in vitro culture, encapsulated microcuttings; in vitro culture, micropropagation, propagation; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; Populus tremula × Populus tremuloides; propagules
Received: June 14, 2004; Accepted: December 11, 2005; Published: December 1, 2006 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
Tsvetkov, I., Jouve, L., & Hausman, J.-F. (2006). Effect of alginate matrix composition on regrowth of in vitro-derived encapsulated apical microcuttings of hybrid aspen. Biologia plantarum, 50(4), 722-724. doi: 10.1007/s10535-006-0115-0
References
- Attree, S.M., Fowke, L.C.: Embryogeny of gymnosperms: advances in synthetic seed technology of conifers.-Plant Cell Tissue Organ Cult. 35: 1-35, 1993.
Go to original source... - Gardi, T., Picioni, E., Standardi, A.: Effect of bead nutrient composition on regrowth of stored in vitro-derived encapsulated microcuttings of different woody species.-J. Microencapsulation 16: 13-25, 1999. Go to original source...
- Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
- Redenbaugh, K., Paasch, B., Nicol, J., Rossler, M., Viss, P., Walker, R.: Somatic seeds: encapsulation of asexual plant embryos.-Biotechnology 4: 797-801, 1986. Go to original source...
- Sokal, R.R., Rohlf, F.J. (ed.): Biometry: The Principles and Practice of Statistics in Biological Research.-W.H. Freeman & Co., New York 1994.
- Standardi, A., Piccioni, E.: Recent perspectives of synthetic seed technology using non-embryogenic in vitro-derived explants.-Int. J. Plant Sci. 159: 968-978, 1998. Go to original source...
- Uozumi, N., Kobayashi, T.: Artificial seed production through encapsulation of hairy roots and shoot tips-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 30. Somatic Embryogenesis and Synthetic Seed. Pp. 170-180. Springer, Berlin 1995. Go to original source...