biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:725-728, 2006 | DOI: 10.1007/s10535-006-0116-z

Establishment of Camptotheca acuminata regeneration from leaf explants

H. -M. Wang1, Y. -G. Zu1,*, W. -J. Wang1, S. -X. Wu1, F. -L. Dong1
1 Key Laboratory of Forest Plant Ecology, the Ministry of Education, Northeast Forestry University, Harbin, China

Plantlet regeneration through shoot formation from young leaf explant-derived callus of Camptotheca acuminata is described. Calli were obtained by placing leaf explants on Woody plant medium (WPM) supplemented with various concentrations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Callus induction was observed in all media evaluated. On the shoot induction medium, the callus induced on the WPM medium containing 19.8 μM BA and 5.8 μM NAA was the most effective, providing high shoot regeneration frequency (70.3 %) as well as the highest number of shoots (11.2 shoots explant-1). The good rooting percentage and root quality (98 %, 5.9 roots shoot-1) were achieved on WPM medium supplemented with 9.6 μM indole-3-butyric acid (IBA). 96 % of the in vitro rooted plantlets with well developed shoots and roots survived transfer to soil.

Keywords: callus; ex vitro transfer; phytohormones; rooting; shoot regeneration
Subjects: auxins; Camptotheca acuminata; cytokinins; ex vitro transfer; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Woody plant medium (WPM); root, rooting

Received: October 12, 2004; Accepted: September 1, 2005; Published: December 1, 2006  Show citation

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Wang, H.-M., Zu, Y.-G., Wang, W.-J., Wu, S.-X., & Dong, F.-L. (2006). Establishment of Camptotheca acuminata regeneration from leaf explants. Biologia plantarum, 50(4), 725-728. doi: 10.1007/s10535-006-0116-z
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