biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 50:729-732, 2006 | DOI: 10.1007/s10535-006-0117-y

Direct shoot organogenesis and plant regeneration in Fortunella crassifolia

L. Yang1, C. -J. Xu1, G. -B. Hu2, K. -S. Chen1,*
1 The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Huajiachi Campuss, Zhejiang University, Hangzhou, P.R. China
2 Department of Fruit Science, South China Agricultural University, Guangzhou, P.R. China

An efficient in vitro regeneration system in kumquats (Fortunella crassifolia Swingle) was established. Explant types and orientations, concentrations and combinations of plant growth regulators were evaluated for their influences on efficiency of plant regeneration. It was found that the optimum explant and its orientation was epicotyl planted vertically with upper part upward, and a shoot regeneration frequency of 1.48 shoots per explant was obtained on Murashige and Skoog (1962; MS) medium supplemented with 22.19 μM 6-benzyladenine (BA). A rooting percentage as high as 74 % was obtained on 1/2 MS supplemented with 0.54 μM 1-naphthaleneacetic acid (NAA), 9.29 μM kinetin (KN), and 0.5 g dm-3 activated charcoal.

Keywords: auxins; cotyledon; cytokinins; epicotyl; explant; hypocotyl; kumquat; rooting
Subjects: cotyledon; cytokinins; epicotyl; Fortunella crassifolia; hypocotyl; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; root, rooting

Received: October 19, 2004; Accepted: October 18, 2005; Published: December 1, 2006  Show citation

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Yang, L., Xu, C.-J., Hu, G.-B., & Chen, K.-S. (2006). Direct shoot organogenesis and plant regeneration in Fortunella crassifolia. Biologia plantarum, 50(4), 729-732. doi: 10.1007/s10535-006-0117-y
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    References

    1. Al-Bahrany, A.A.: Effect of phytohormones on in vitro shoot multiplication and rooting of lime Citrus aurantifolia (Christm.) Swing.-Sci. Hort. 95: 285-295, 2002. Go to original source...
    2. Beloualy, N.: Plant-regeneration from callus-culture of 3 citrus rootstocks.-Plant Cell Tissue Organ Cult. 24: 29-34, 1991. Go to original source...
    3. Bordón, Y., Guardiola, J.L., García-Luis, A.: Genotype affects the morphorgenic response in vitro of epicotyl segments of Citrus rootstocks.-Ann. Bot. 86: 159-166, 2000. Go to original source...
    4. Chen, D.-M., Xu, C.-J., Zhang, S.-L.: [Studies on a direct shoot regeneration system of Citrus sinensis L. Osbeck with a high frequency in vitro].-J. Zhejiang Univ. 26: 493-499, 2000. [In Chin.]
    5. Costa, M.G.C., Alves, V.S., Lani, E.R.G., Mosquima, P.R., Carvalho, C.R., Otoni, W.C.: Morphogenic gradients of adventitious bud and shoot regeneration in epicotyl explants of Citrus.-Sci. Hort. 100: 63-74, 2004. Go to original source...
    6. Ghorbel, R., Domínguez, A., Navarro, L., Peña, L.: High efficiency genetic transformation of sour orange (Citrus aurantium) and production of transgenic trees containing the coat protein gene of citrus tristeza virus.-Tree Physiol. 20: 1183-1189, 2000. Go to original source...
    7. Hassanein, A.M., Azooz, M.M.: Propagation of Citrus reticulata via in vitro seed germination and shoot cuttings.-Biol. Plant. 47: 173-177, 2003. Go to original source...
    8. He, H., Pan, R.-C., He, Y.-W., Han, M.-L., Li, G.-G.: [A study on the tissue culture and plant regeneration of Citrus reticulata cv. Tankan].-J. South China norm. Univ. (nat. Sci. Ed.) 1997(4): 63-66, 1997. [In Chin.]
    9. Hiregoudar, L.V., Ashok Kumar, H.G., Murthy, H.N.: In vitro culture of Feronia limonia (L.) Swingle from hypocotyl and internodal explants.-Biol. Plant. 49: 41-45, 2005. Go to original source...
    10. Huang, T., Peng, S.-L., Dong, G.-F., Zhang, L.-Y., Li, G.-G.: Plant regeneration from leaf-derived callus in Citrus grandis (pummelo): effects of auxins in callus induction medium.-Plant Cell Tissue Organ Cult. 69: 141-146, 2002.
    11. Jia, Y.-J., Chen, F., Lin, H.-H., Cao, Y.-L., Luo, Y.-Y.: [In vitro culture of different explant from Fortunella margarita (Lour.) Swing].-J. Sichuan Univ. (nat. Sci. Ed.) 34: 344-348, 1997. [In Chin.]
    12. Ling, J.T., Iwamasa, M.: Plant regeneration from embryogenic calli of six Citrus related genera.-Plant Cell Tissue Organ Cult. 49: 145-148, 1997. Go to original source...
    13. Murashige, T., Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    14. Singh, S., Ray, B.K., Bhattacharyya, S., Deka, P.C.: In vitro propagation of Citrus reticulata Blanco and Citrus limon Burm.f.-HortScience 29: 214-216, 1994. Go to original source...
    15. Wong, W.-S., Li, G.-G., Ning, W., Xu, Z.-F., Hsiao, W.L.W., Zhang, L.-Y., Li, N.: Repression of chilling-induced ACC accumulation in transgenic citrus by over-production of antisense 1-aminocyclopropane-1-carboxylate synthase RNA.-Plant Sci. 161: 969-977, 2001. Go to original source...

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