biologia plantarum

Biologia plantarum 50:733-736, 2006 | DOI: 10.1007/s10535-006-0118-x

Rapid micropropagation of mature wild cherry

J. Ďurkovič¹

¹ Department of Phytology, Technical University, Zvolen, Slovak Republic

Explants taken from the mature vigorous tree of wild cherry (Prunus avium L.) were assayed for their organogenic capacity under various phytohormonal treatments. The highest rate of adventitious shoot multiplication was recorded at a combination of 0.5 mg dm^-3 6-benzylaminopurine (BAP) and 0.05 mg dm^-3 thidiazuron (6.83 shoots per explant). No differences in multiplication rates were found among media supplemented with BAP, BAP + α-naphthaleneacetic acid (NAA) or BAP + indole-3-butyric acid (IBA). Shoot elongation was significantly affected by the concentration of BAP, regardless of auxin addition to medium. Up to 73 % of microshoots rooted after using 0.3 mg dm^-3 IBA, otherwise the adventitious rooting occurred at reasonable frequencies in all auxin treatments. Regenerated plantlets were successfully hardened ex vitro and continued to grow after the transfer to soil. No morphological aberrations were observed in the regenerates.

Keywords: adventitious shoot multiplication; Prunus avium; thidiazuron

Subjects: auxins; cytokinins; in vitro culture, micropropagation, propagation; in vitro culture, regeneration, proliferation, differentiation; Prunus avium; thidiazuron (TDZ); wild cherry

Received: October 20, 2004; Accepted: May 24, 2005; Published: December 1, 2006  Show citation