biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:741-744, 2006 | DOI: 10.1007/s10535-006-0120-3

Efficient plant regeneration from shoot apices of sorghum

M. Maheswari1,*, N. Jyothi Lakshmi1, S. K. Yadav1, Y. Varalaxmi1, A. Vijaya Lakshmi1, M. Vanaja1, B. Venkateswarlu1
1 Division of Crop Sciences, Central Research Institute for Dryland Agriculture, Santosh Nagar, Hyderabad, India

An efficient and rapid regeneration protocol was developed using shoot apices from germinating seedlings of two cultivars of sorghum, SPV-462 and M35-1, as explants. A vertical slit given from the base of each dissected apex enhanced the efficiency of callusing response by two fold. MS medium containing 0.5 mg dm-3 each of 2,4-D and kinetin was most effective in producing friable and embryogenic calli. Scanning electron microscopy of these calli detected somatic embryogenesis. Calli thus induced gave rise to approximately 42 green shoots per callus in both the genotypes when transferred to regeneration medium containing 1.5 mg dm-3 kinetin.

Keywords: 2,4-dichlorophenoxyacetic acid; kinetin; plant growth regulators; Sorghum bicolor (L.) Moench
Subjects: auxins; cytokinins; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; rapid regeneration; root, rooting; sorghum; Sorghum bicolor

Received: December 6, 2004; Accepted: October 1, 2005; Published: December 1, 2006  Show citation

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Maheswari, M., Jyothi Lakshmi, N., Yadav, S.K., Varalaxmi, Y., Vijaya Lakshmi, A., Vanaja, M., & Venkateswarlu, B. (2006). Efficient plant regeneration from shoot apices of sorghum. Biologia plantarum, 50(4), 741-744. doi: 10.1007/s10535-006-0120-3
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