biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:755-758, 2006 | DOI: 10.1007/s10535-006-0124-z

Identification of RAPD markers linked to the karnal bunt resistance genes in wheat

M. Kumar1,*, O. P. Luthra1, V. Chawla1, L. Chaudhary4, N. Saini1, A. Poonia1, R. Kumar2, A. P. Singh3
1 Department of Genetics, Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar, India
2 Department of Plant Physiology, CCS Haryana Agricultural University, Hisar, India
3 Department of Biochemistry, CCS Haryana Agricultural University, Hisar, India
4 Department of Plant Breeding, GB Pant University of Agriculture and Technology, Pantnagar, India

A set of 104 wheat recombinant inbred lines (RILs) obtained from a cross between parents resistant (HD 29) and susceptible (WH 542) to karnal bunt (KB) (caused by Neovossia indica) were screened and used to identify random amplified polymorphic DNA (RAPD) markers linked with resistance to karnal bunt as these would allow indirect marker assisted selection of KB resistant genotypes. The two parents were analysed with 92 RAPD primers. A total of 65 primers proved functional by giving scorable polymerase chain reaction (PCR) products. Of these, 21 (32 %) primers detected polymorphism between the two parental genotypes. Using these primers, bulked segregant analysis was carried out on two bulk DNAs, one obtained by pooling DNA from 10 KB resistant RILs and the other similarly derived by pooling 10 KB susceptible RILs. One marker, OPM-20 showed apparent association with resistance to KB. This was confirmed following selective genotyping of individual RILs included in the bulks.

Keywords: Neovossia indica; PCR; recombinant inbred lines; Triticum aestivum
Subjects: karnal bunt; Neovossia indica; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD); recombinant inbred lines; Triticum aestivum

Received: January 21, 2005; Accepted: August 19, 2005; Published: December 1, 2006  Show citation

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Kumar, M., Luthra, O.P., Chawla, V., Chaudhary, L., Saini, N., Poonia, A., Kumar, R., & Singh, A.P. (2006). Identification of RAPD markers linked to the karnal bunt resistance genes in wheat. Biologia plantarum, 50(4), 755-758. doi: 10.1007/s10535-006-0124-z
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