biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:759-762, 2006 | DOI: 10.1007/s10535-006-0125-y

Protein profile in the transgenic kiwifruit overexpressing a transcription factor gene, OSH1

M. Kita1,*, C. Honda1, S. Komatsu2, S. Kusaba3, Y. Fujii4, T. Moriguchi1
1 National Institute of Fruit Tree Science, Tsukuba, Ibaraki, Japan
2 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
3 National Agricultural Research Center for Western Region, Zentsuji, Kagawa, Japan
4 National Institute of Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan

The aim of this study was to investigate the protein alterations in the transgenic kiwifruit (Actinidia deliciosa) overexpressing a transcription factor gene, OSH1. Although transgenic plant with introduced OSH1 indicates suppression of gibberellin (GA)-20 oxidase activity, application of GA to transgenic kiwifruit could not completely recover plant morphology and protein profiles. Eleven proteins decreased in the transgenic kiwifruit detected by two-dimensional polyacrylamide gel electrophoresis showed homologies to kiwifruit hypothetical protein, osmotin I and photosynthesis related protein. These results suggest that introduction of an OSH1 into kiwifruit caused wide-range alterations at protein level and alterations of protein accumulation should be considered to evaluate the substantial equivalence of plants transformed by a transcription factor.

Keywords: Actinidia deliciosa; 2D-PAGE; gibberellin oxidase; gibberellins; proteomics
Subjects: Actinidia deliciosa; gibberellic acid, gibberellins; gibberellin oxidase; kiwifruit; osmotin; proteins; transgenic plants (cf. also in vitro…)

Received: January 28, 2005; Accepted: August 8, 2005; Published: December 1, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kita, M., Honda, C., Komatsu, S., Kusaba, S., Fujii, Y., & Moriguchi, T. (2006). Protein profile in the transgenic kiwifruit overexpressing a transcription factor gene, OSH1. Biologia plantarum, 50(4), 759-762. doi: 10.1007/s10535-006-0125-y
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Celis, J.E., Bravo R. (ed): Two-Dimensional Gel Electrophoresis of Proteins Methods and Applications.-Academic Press, New York 1984.
    2. Cleveland, D.W., Fischer, S.G., Kirschner, M.W., Laemmeli, U.K.: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.-J. biol. Chem. 252: 1102-1106, 1977. Go to original source...
    3. Damerval, C., Vienne, D., Zivy, M., Thiellement, H.: Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins.-Electrophoresis 7: 52-54, 1986. Go to original source...
    4. Dell'Aquila, A.: Protein patterns, characterized by computer image analysis, of lentil embryo axes germinating under salt stress.-Biol. Plant. 48: 237-242, 2004. Go to original source...
    5. Gehring, W.J.: Homeoboxes in the study of development.-Science 236: 1245-1252, 1987. Go to original source...
    6. Gygi, S.P., Corthals, G.L., Zhang, Y., Rochon, Y., Aebersold, R.: Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.-Proc. nat. Acad. Sci. USA 97: 9390-9395, 2000. Go to original source...
    7. Kano-Murakami, Y., Yanai, T., Tagiri, A. Matsuoka, M.: A rice homeotic gene, OSH1, causes unusual phenotypes in transgenic tobacco.-FEBS Lett. 334: 365-368, 1993. Go to original source...
    8. Komatsu, S., Yang, G., Unno, K., Park, P.: Characterization of a membrane-associated phosphoprotein (pp47) in rice (Oryza sativa L.) seedlings treated by gibberellin.-J. Plant Physiol. 159: 121-128, 2002. Go to original source...
    9. Konishi, H., Ishiguro, K., Komatsu, S.: A proteomics approach towards understanding blast fungus infection of rice grown under different levels of nitrogen fertilization.-Proteomics 1: 1162-1171, 2001. Go to original source...
    10. Kusaba, S., Fukumoto, M., Honda, C., Yamaguchi, I., Sakamoto, T. Kano-Murakami, Y.: Decreased GA1 content caused by the overexpression of OSH1 is accompanied by suppression of GA20-oxidase gene expression.-Plant Physiol. 117: 1179-1184, 1998. Go to original source...
    11. Kusaba, S., Kano-Murakami, Y., Matsuoka, M., Matsuta, N., Sakamoto, T., Fukumoto, M.: Expression of the rice homeobox gene, OSH1, causes morphological changes in transgenic kiwifruit.-J. jap. Soc. hort. Sci. 68: 482-486, 1999. Go to original source...
    12. Ledger, S.E., Gardner, R.C.: Cloning and characterization of five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var. deliciosa).-Plant mol. Biol. 25: 877-886, 1994. Go to original source...
    13. Marenholz, I., Zirra, M., Fischer, D.F., Backendorf, C., Ziegler, A., Mischke, D.: Identification of human epidermal differentiation complex (EDC)-encoded genes by subtractive hybridization of entire YACs to a gridded keratinocyte cDNA library.-Genome Res. 11: 341-355, 2001. Go to original source...
    14. Matsuoka, M., Tamaoki, M., Tada, Y., Fuyjimura, T., Tagiri, A., Yamamoto, N., Kano-Murakami, Y.: Expression of rice OSH1 gene is localized in developing vascular strands and its ectopic expression in transgenic rice causes altered morphology of leaf.-Plant Cell Rep. 14: 555-559, 1995. Go to original source...
    15. O'Farrell, P.H.: High resolution two-dimensional electrophoresis of proteins.-J. biol. Chem. 250: 4007-4021, 1975. Go to original source...
    16. Sakamoto, T., Kamiya, N., Ueguchi-Tanaka, M., Iwahori, S., Matsuoka, M.: KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem.-Gene Dev. 15: 581-590, 2001. Go to original source...
    17. Scott, M.P., Tamkun, J.W., Hartzell, G.W., III.: The structure and function of the homeodomain.-Biochem. biophys. Acta 989: 25-48, 1989. Go to original source...
    18. Singh, N.K., Bracker, C.A., Hasegawa, P.M., Handa, A.K., Buckel, S., Hermodson, M.A., Pfankoch, E., Regnier, F.E., Bressan, R.A.: Characterization of osmotin, a thaumatin-like protein associated with osmotic adaptation in plant cells.-Plant Physiol. 85: 529-536, 1987. Go to original source...
    19. Tamaoki, M., Yamamoto, N., Matsuoka, M.: Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1.-Plant Cell Physiol. 38: 638-642, 1997. Go to original source...

    Return to the content