biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:782-784, 2006 | DOI: 10.1007/s10535-006-0131-0

Effects of carbon sources and auxins on in vitro propagation of banana

P. Madhulatha1, S. I. Kirubakaran1, N. Sakthivel1,*
1 Department of Biotechnology, Pondicherry University, Pondicherry, India

The effects of carbon sources (sucrose, glucose, fructose and mannitol) and auxins [indolebutyric acid (IBA) and α-naphthaleneacetic acid (NAA)] on in vitro propagation of banana (Musa spp. AAA) were studied. Over all carbon sources tested, sucrose induced highest frequency of shoot proliferation. Optimal shoot proliferation rates were achieved on the Murashige and Skoog (MS) medium supplemented with sucrose and glucose combination (1:1) at the concentration of 30 g dm-3. Similarly, higher frequency of root induction was obtained at IBA and NAA combination (1:1; concentration of 2 mg dm-3) than at other concentrations of IBA or NAA alone or their combinations.

Keywords: carbon sources; auxins; banana (Musa spp. AAA); in vitro propagation
Subjects: auxins; banana; fructose; in vitro culture, micropropagation, propagation; Musa sp.; nutrient medium, Murashige and Skoog; sucrose; sugars

Received: March 1, 2005; Accepted: April 18, 2005; Published: December 1, 2006  Show citation

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Madhulatha, P., Kirubakaran, S.I., & Sakthivel, N. (2006). Effects of carbon sources and auxins on in vitro propagation of banana. Biologia plantarum, 50(4), 782-784. doi: 10.1007/s10535-006-0131-0
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