biologia plantarum

Biologia plantarum 51:27-33, 2007 | DOI: 10.1007/s10535-007-0006-z

Development of the CELLOP optimisation model for plant cell cultivation

M. Eeva¹, P. Vuorela², P. Tammela³, M. Nyman⁴, S. Ojala¹, K. Fagerstedt⁴, H. Haario⁵, H. Vuorela^1,*

¹ Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Helsinki, Helsinki, Finland

² Department of Biochemistry and Pharmacy, Biocity, Åbo Akademi University, Turku, Finland

³ Drug Discovery and Development Technology Center, Faculty of Pharmacy, University of Helsinki, Finland

⁴ Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, Finland

⁵ Department of Mathematics, University of Helsinki, Finland

A mathematical computer-aided model CELLOP was constructed in which the desirability functions in a three-dimensional experimental design can be used to find the optimal growing conditions for plant cells. CELLOP is intended for the optimisation of 3 to 6 physical, chemical, or biological variables in the cultivation conditions of plant cell cultures. The model was used to optimise the culturing conditions (calcium, inorganic nitrogen, and sucrose concentrations) for coumarin-producing, spontaneously embryogenic cell lines of angelica Angelica archangelica L. subsp. archangelica and hogs fennel Peucedanum palustre (L.) Moench. For A. archangelica the overall optimum concentrations were 0.47 mM Ca²⁺, 5.06 mM NO₃ ^-, 0.40 mM NH₄ ⁺, and 96.25 mM sucrose. The dry mass was 24.7 % higher and the coumarin content 40.5 % higher than those achieved in the standard 75 % Gamborg B5 medium. For A. archangelica the highest embryogenic activity was reached in the media containing 1.25 mM Ca²⁺. In the case of P. palustre the overall optimum concentrations were 1.60 mM Ca²⁺, 2.84 mM NO³ _-, 0.23 mM NH₄ ⁺, and 85.10 mM sucrose. For P. palustre the dry mass production increased by 61.8 % and the coumarin content by 58.1 % compared to the values achieved in the Gamborg B5 medium. For P. palustre the highest embryogenic activity was reached in the presence of 50.0 mM NO₃ ^- and 4.01 mM NH₄ ⁺.

Keywords: Angelica archangelica; desirability function; embryogenic cell lines; Peucedanum palustre

Subjects: Angelica archangelica; coumarin; hogs fennel; in vitro culture, embryogenesis, somatic embryogenesis; model CELLOP; model PRISMA; nutrient medium, Murashige and Skoog (MS); Paucedanum palustre

Received: November 12, 2004; Accepted: March 14, 2006; Published: March 1, 2007  Show citation