biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 51:169-172, 2007 | DOI: 10.1007/s10535-007-0033-9

Molecular characterization of Mauritanian date palm cultivars using plasmid-like DNAs markers

A. Ould Mohamed Salem1,*, S. Rhouma2, S. Zehdi2, M. Marrakchi2, M. Trifi2
1 Département de Biologie, Faculté des Sciences et Techniques, Université de Nouakchott, Mauritania
2 Faculté des Sciences de Tunis, Campus Universitaire, El Manar Tunis, Tunisia

Mauritanian date palm cultivars and progenies of two controlled crosses were analyzed according to the identity of mitochondrial plasmid-like DNAs. Starting from total genomic DNA and appropriate primers, polymerase chain reaction was designed to amplify either a 373-bp or a 265-bp fragments corresponding to the S and the R-plasmid respectively. Data proved that 5 cultivars out of 10 studied have exhibited the R-plasmid suggesting their resistance to the fusariosis. The existence of intra-cultivar variability has also been revealed in the cv. Ahmar. In addition, analysis throughout progenies of two controlled crosses suggested the strict maternal transmission of the date palms' mitochondrial genome.

Keywords: Phoenix dactylifera L; fusariosis; genetic inheritance; PCR
Subjects: cultivar and genotype differences; date palm; DNA markers; fusariosis; Phoenix dactylifera; polymerase chain reaction (PCR)

Received: November 16, 2004; Accepted: February 21, 2006; Published: March 1, 2007  Show citation

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Ould Mohamed Salem, A., Rhouma, S., Zehdi, S., Marrakchi, M., & Trifi, M. (2007). Molecular characterization of Mauritanian date palm cultivars using plasmid-like DNAs markers. Biologia plantarum, 51(1), 169-172. doi: 10.1007/s10535-007-0033-9
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