biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:193-197, 2007 | DOI: 10.1007/s10535-007-0039-3

Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris

A. E. Valdés1, P. Galuszka2, B. Fernández1,*, M. L. Centeno3, I. Frébort2
1 Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, Oviedo, Spain
2 Faculty of Science, Palacký University, Olomouc, Czech Republic
3 Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain

In the present study cytokinin dehydrogenase (CKX) activity was for the first time found in a conifer species, Pinus sylvestris. The activities were correlated with the endogenous cytokinin contents. Several enzyme substrates and two different electron acceptors were used to search for the enzyme activity in the extract from seeds, seedlings and plantlets. The highest specific activity was found in one-year-old plantlets with isopentenyladenine as the substrate and 2,6-dichlorophenolindophenol as the electron acceptor, at pH 8. An enhancement in the CKX specific activity corresponded to increasing contents of cytokinins, mainly isopentenyladenine and isopentenyladenosine, indicating that the enzyme activity is affected by the endogenous supply of cytokinins. CKX affinity for the ribosylated form of isopentenyladenine was dependent on the developmental stage, being higher in seeds than in seedlings, and not detectable in plantlets. The results are indicative of the presence of different isoenzymes throughout the development.

Keywords: cytokinin metabolism; conifers; plant ageing
Subjects: cytochrome c oxidase; cytokinin oxidase/dehydrogenase; cytokinins; growth analysis, plant development, biomass and yield enhancement; Pinus sylvestris

Received: May 11, 2005; Accepted: October 31, 2005; Published: March 1, 2007  Show citation

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Valdés, A.E., Galuszka, P., Fernández, B., Centeno, M.L., & Frébort, I. (2007). Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris. Biologia plantarum, 51(1), 193-197. doi: 10.1007/s10535-007-0039-3
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