biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 51:198-200, 2007 | DOI: 10.1007/s10535-007-0040-x

Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species

K. Wotavová-Novotná1,*, H. Vejsadová2, P. Kindlmann1
1 Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Èeské Budìjovice, Czech Republic
2 Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Prùhonice, Czech Republic

The influence of sugars and growth regulators on shoot and root growth of Dactylorhiza species was studied under in vitro conditions. The seedling development was stimulated with the application of glucose and sucrose at concentration of 10 g dm-3 each. The improvement of shoot growth rate and shoot length was enhanced by cytokinins N 6-(2-isopentenyl)adenine or N 6-benzyladenine and their combination with auxin indolebutyric acid (IBA). The root growth rate and root length of seedlings increased in the presence of IBA and α-naphthaleneacetic acid. Individual Dactylorhiza species showed statistically significant differences in shoot and root development depending on sugar and growth regulator combinations.

Keywords: auxins; cytokinins; glucose; in vitro cultivation; sucrose; terrestrial orchids
Subjects: auxins; cytokinins; Dactylorhiza sp.; in vitro culture, growth, cultivation; orchids; sucrose; sugars

Received: September 16, 2005; Accepted: May 25, 2006; Published: March 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wotavová-Novotná, K., Vejsadová, H., & Kindlmann, P. (2007). Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species. Biologia plantarum, 51(1), 198-200. doi: 10.1007/s10535-007-0040-x
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arditti, J., Ernst, R., Yam, T.W., Gable, C.: The contribution of orchid mycorrhizal fungi to seed germination: a speculative review.-Lindleyana 5: 249-255, 1990.
    2. De Pauw, M.A., Remphrey, W.R., Palmer C.E.: The cytokinin preference for in vitro germination and protocorm growth of Cypripedium candidum.-Ann. Bot. 75: 267-275, 1995. Go to original source...
    3. Hadley, G.: The interaction of kinetin, auxin and other factors in the development of north temperate orchids.-New Phytol. 69: 549-555, 1970. Go to original source...
    4. Harvais, G.: Growth requirements and development of Cymbidium reginae in axenic culture.-Can. J. Bot. 51: 327-332, 1973. Go to original source...
    5. Harvais, G., Hadley, G.: The development of Orchis purpurella in asymbiotic and inoculated cultures.-New Phytol. 66: 217-230, 1967. Go to original source...
    6. Masuhara, G., Katsuya, K.: In situ and in vitro specificity between Rhizoctonia spp. and Spiranthes sinensis (Persoon) Ames. var. amoena (M. Bieberstein) Hara (Orchidaceae).-New Phytol. 127: 711-718, 1994. Go to original source...
    7. Michl, J.: [Growing and propagation of European orchids.]-Roezliana 12: 2941, 1981. [In Czech.]
    8. Pritchard, H.W., Prendergast, F.G.: Factor influencing the germination and storage characteristics of orchid pollen.-In: Pritchard, H.W. (ed.): Modern Methods in Orchid Conservation. Pp. 1-16. Cambridge University Press, Cambridge 1989. Go to original source...
    9. Rasmussen, H.: Terrestrial Orchids: from Seed to Mycotrophic Plant.-Cambridge University Press, Cambridge 1995. Go to original source...
    10. Scott, H.S., Carey, P.D.: The effects of fungicide and water application on seed germination and infection in Gymnadenia conopsea under field conditions.-In: Kindlmann, P., Whigham, D.F., Willems, J.H. (ed.): Trends and fluctuations, and underlying mechanisms in terrestrial orchid populations. Pp. 155-165. Backhuys Puiblishing, Leiden 2002.
    11. Smith, S.E., Read, D.J. (ed.): Mycorrhizal Symbiosis.-Academic Press, London 1997.
    12. Stewart, J.: Orchid propagation by tissue culture - past, present and future.-In: Pritchard, H.W. (ed.): Modern Methods in Orchid Conservation. Pp. 87-102. Cambridge University Press, Cambridge 1989. Go to original source...
    13. Van Waes, J.M, Debergh, P.C.: In vitro germination of some Western European orchids.-Physiol. Plant. 67: 253-261, 1986. Go to original source...
    14. Zettler, L.W., Hofer, C.J.: Propagation of the little club-spur orchid (Platanthera clavellata) by symbiotic seed germination and its ecological implications.-Environ. exp. Bot. 39: 189-195, 1998. Go to original source...

    Return to the content