biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:235-241, 2007 | DOI: 10.1007/s10535-007-0047-3

Ginsenoside production, growth and cytogenetic characteristics of sustained Panax japonicus var. Repens cell suspension culture

I. N. Smolenskaya1,*, O. V. Reshetnyak1, A. V. Nosov1, S. E. Zoriniants1, A. L. Chaiko2, Y. N. Smirnova1, A. M. Nosov1
1 Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
2 Institute of Food Chemistry and Technology, Ukrainian National Academy of Sciences and Ministry of Agricultural Politics, Kyiv, Ukraine

Cytophysiological and cytogenetic characteristics of cell suspension culture of Panax japonicus var. repens were studied in relation to the accumulation of ginsenosides (GSs). The minimal time of cell number doubling was 1.3 ± 0.1 d and cell number increased 7 to 8-fold during growth cycle. The cell culture can be considered as aneuploid with about tetraploid (46-60 chromosomes) modal class. Upon long-term cultivation, the total content of GSs considerably increased and maximal concentration of GSs was 2.2 %(d.m.). The ratio of seven major GSs only slightly altered both over each and different subcultures. The overall amount of GSs of Rg-group significantly exceeded that of Rb-group. Cell volume and the number of large cellular aggregates with the higher proportion (by 20 %) of parenchymal cells increased late in the subculture. In this time the population contained about 20 % of the cells with doubled amount of nuclear DNA and accompanied with elevation in the GS content. These data prompted us to suggest that biosynthesis of GSs has a link with cell differentiation.

Keywords: chromosome number; cytodifferentiation; ginseng; in vitro culture; nuclear DNA content; triterpenes
Subjects: ginsenoside; Panax japonicus

Received: May 20, 2004; Accepted: January 3, 2006; Published: June 1, 2007  Show citation

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Smolenskaya, I.N., Reshetnyak, O.V., Nosov, A.V., Zoriniants, S.E., Chaiko, A.L., Smirnova, Y.N., & Nosov, A.M. (2007). Ginsenoside production, growth and cytogenetic characteristics of sustained Panax japonicus var. Repens cell suspension culture. Biologia plantarum, 51(2), 235-241. doi: 10.1007/s10535-007-0047-3
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