biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 51:327-332, 2007 | DOI: 10.1007/s10535-007-0063-3

An elicitor-and pathogen-induced cdna from potato encodes a stress-responsive cyclophilin

I. A. Dubery1,*
1 Department of Biochemistry, University of Johannesburg, Auckland Park 2006, South Africa

Differential mRNA display was used to identify pathogen-responsive, stress-related genes in potato cell suspensions treated with salicylic acid and a cell wall-derived elicitor from Phytophthora infestans. Among the positive clones identified, one was found to be expressed at a significantly higher level in elicited cells than in control cells. DNA sequencing of this amplicon revealed high homology and identified it as a potato cyclophilin cDNA. The maximum amount of the cyclophilin mRNA was found 9 to 12 h after elicitation. Cyclophilin (CyP) mRNA synthesis was also up-regulated from 12 to 24 h in potato leaves locally infected with zoospores from Phytophthora infestans. However, untreated leaves responding systemically to the pathogen showed only a weak, delayed response at 24 h post infection. The observed accumulation of potato CyP mRNA in response to salicylic acid, P. infestans elicitor and P. infestans infection, suggest that CyPs play an important role in plant stress responses.

Keywords: chaperone; Phytophthora infestans; Solanum tuberosum; stress response
Subjects: chaperones; cyclophilin; elicitors; Phytophthora infestans; potato

Received: March 31, 2005; Accepted: February 21, 2006; Published: June 1, 2007  Show citation

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Dubery, I.A. (2007). An elicitor-and pathogen-induced cdna from potato encodes a stress-responsive cyclophilin. Biologia plantarum, 51(2), 327-332. doi: 10.1007/s10535-007-0063-3
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