biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:363-366, 2007 | DOI: 10.1007/s10535-007-0075-z

Localization of cadmium in the root cells of Allium cepa by energy dispersive x-ray analysis

D. Liu1,*, I. Kottke2, D. Adam3
1 Department of Biology, College of Chemistry and Life Sciences, Tianjin Normal University, Tianjin, P.R. China
2 Botanisches Institut, Eberhard-Karls Universität Tübingen, Tübingen, Germany
3 Institut für Angewandte Physik, Eberhard-Karls Universität Tübingen, Tübingen, Germany

Allium cepa L. roots were exposed to 0.1 and 1.0 mM Cd for 6, 24 and 48 h and the localization of Cd in the root tissue was investigated. Scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDXA) were performed on frozen-dried tissues of roots. No Cd was detected in the roots treated with only 0.1 mM Cd, while after exposure to higher Cd concentration (1.0 mM) Cd was observed in cell wall and in cytoplasm in the epidermis, cortex and vascular tissues in the roots.

Keywords: cell wall; cytoplasm; scanning electron microscopy (SEM)
Subjects: cadmium; cell wall; energy dispersive X-ray analysis; heavy metals; in vitro culture, root cultivation, rooting; metals; scanning electron microscopy

Received: August 15, 2005; Accepted: April 27, 2006; Published: June 1, 2007  Show citation

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Liu, D., Kottke, I., & Adam, D. (2007). Localization of cadmium in the root cells of Allium cepa by energy dispersive x-ray analysis. Biologia plantarum, 51(2), 363-366. doi: 10.1007/s10535-007-0075-z
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