biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:367-371, 2007 | DOI: 10.1007/s10535-007-0077-x

Soapwort oxidoreductase is involved in trinitrotoluene detoxification

R. Podlipná1, A. Nepovím1, P. Soudek1, M. Vágner2, T. Vaněk1,*
1 Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Praha 6, Czech Republic
2 Laboratory of Biologically Active Compounds, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic

Plant enzymes participating in degradation of nitroaromatic compounds have not been biochemically characterized in details so far. From suspension culture of soapwort (Saponaria officinalis L.) we isolated a novel plant oxidoreductase involved in degradation of trinitrotoluene (TNT). The enzyme catalyses first steps of reduction of TNT nitro groups in the presence of NAD(P)H under anaerobic conditions. The enzyme is monomeric with molecular mass 29 kDa, its two isoforms have pI 4.8 and 5.1. According to the spectral and activation analysis the enzyme contains flavinmono-nucleotide as a prosthetic group. The structure properties suggest an evolutional relationship to oxophytodienoate reductase. The N-terminal amino acid sequence shows homology to family of Old Yellow Enzyme (E.C. 1.6.99.1).

Keywords: flavoprotein; Old Yellow Enzyme; oxidoreductase; phytoremediation; Saponaria officinalis
Subjects: cytokinins; nutrient medium, Murashige and Skoog (MS); oxidoreductases; phytoremediation; Saponaria officinalis; soapwort

Received: December 7, 2005; Accepted: March 25, 2006; Published: June 1, 2007  Show citation

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Podlipná, R., Nepovím, A., Soudek, P., Vágner, M., & Vaněk, T. (2007). Soapwort oxidoreductase is involved in trinitrotoluene detoxification. Biologia plantarum, 51(2), 367-371. doi: 10.1007/s10535-007-0077-x
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