biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:391-394, 2007 | DOI: 10.1007/s10535-007-0084-y

Activity of secreted cell wall-modifying enzymes and expression of peroxidase-encoding gene following germination of Orobanche ramosa

C. Veronesi1,*, E. Bonnin2, S. Calvez3, P. Thalouarn1, P. Simier1
1 Groupe de Physiologie et Pathologie Végétales, Faculté des Sciences et Techniques, Université de Nantes, Nantes Cedex 3, France
2 Biopolymères-Interactions-Assemblage, INRA, Nantes Cedex 3, France
3 Unité de Recherche QM2A, ENITIAA, Nantes Cedex 3, France

Radicle growth of germinated seed of the root parasite O. ramosa is shown to be rapidly accompanied by secretion of proteins including pectinolytic enzymes, polygalacturonase and rhamnogalacturonase. These secretions peaked between 4 to 8 d after induction of germination and remained constant for some further days in the case of polygalacturonases. After 6 d, germinated seeds secreted proteins which exhibit peroxidase activity. The latter may be correlated with expression of OrPOX1, a putative gene encoding for secreted peroxidase. The involvement of these enzymes in host root attack and haustorium formation by the parasite is discussed.

Keywords: broomrape; cellulase; GR24; hemicellulase; parasitic plants; pectate lyase; stimulants of germination; virulence
Subjects: broomrape; cell wall; cellulase; germination; Orobanche ramose; parasitic plant; pectate lyase; peroxidase; virulence

Received: September 14, 2005; Accepted: May 11, 2006; Published: June 1, 2007  Show citation

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Veronesi, C., Bonnin, E., Calvez, S., Thalouarn, P., & Simier, P. (2007). Activity of secreted cell wall-modifying enzymes and expression of peroxidase-encoding gene following germination of Orobanche ramosa. Biologia plantarum, 51(2), 391-394. doi: 10.1007/s10535-007-0084-y
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