biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:507-513, 2007 | DOI: 10.1007/s10535-007-0107-8

Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus

B. Profotová1, L. Burketová2,*, O. Valentová1
1 Institute of Chemical Technology Prague, Prague, Czech Republic
2 Institute of Experimental Botany CAS, Prague, Czech Republic

Accumulation of extracellular chitinases in Brassica napus plants infected with Turnip yellow mosaic virus (TYMV) and fungal pathogen Leptosphaeria maculans was studied in both compatible and incompatible interaction. Analysis of apoplast fluid by means of non-denaturing anodic and cathodic PAGE followed by in-gel detection of chitinase activity revealed a number of chitinase isozymes. TYMV induced 8 acidic and 4 basic isozymes in a systemic way. Except for one acidic and one basic isozyme, all other chitinases were also constitutively present in low amounts in mock inoculated control. In TYMV systemically infected plants, chitinases were detected in leaves expressing symptoms as well as in symptomless ones. Both virulent and avirulent L. maculans isolates induced production of chitinase isozymes in cotyledons in a time dependent manner. Some of them were present in plants constitutively and their content increased after inoculation. Three of five acidic and two of three basic isozymes responded to L. maculans infection. Chitinases started to accumulate before symptom appearance. First two acidic isozymes were detected 24 h after inoculation. The difference between compatible and incompatibe interaction reflected two basic isozymes.

Keywords: oilseed rape; plant defence; PR-proteins; Turnip yellow mosaic virus
Subjects: Brassica napus; chitinase; cultivar and genotype differences; Leptosphaeria maculans; oilseed rape; plant defence system; proteins, pathogenesis-related (PR-proteins); Turnip yellow mosaic virus (TYMV)

Received: November 14, 2005; Accepted: July 20, 2006; Published: September 1, 2007  Show citation

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Profotová, B., Burketová, L., & Valentová, O. (2007). Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus. Biologia plantarum, 51(3), 507-513. doi: 10.1007/s10535-007-0107-8
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