biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:514-516, 2007 | DOI: 10.1007/s10535-007-0109-6

Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib

A. Subotić1,*, D. Grubišić1
1 Institute for Biological Research Siniša Stanković, Belgrade, Serbia

Direct somatic embryogenesis and adventitious shoot formation were successfully achieved from root explants of Centaurium erythrea Gillib. cultured on Murashige and Skoog medium with half-strength macronutrients, full-strength micronutrients and vitamins, 3 % sucrose, 0.7 % agar, 100 mg dm-3 myo-inositol and without growth regulators. Histological studies revealed that somatic embryos were formed directly from epidermal cells and adventitious buds were developed from meristematic cells in root cortex tissues. Somatic embryos as well as adventitious shoots developed into whole plantlets.

Keywords: histology; organogenesis; root culture
Subjects: Centaurium erythrea; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; in vitro culture, root cultivation, rooting; nutrient medium, Murashige and Skoog (MS)

Received: July 7, 2005; Revised: May 5, 2006; Published: September 1, 2007  Show citation

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Subotić, A., & Grubišić, D. (2007). Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib. Biologia plantarum, 51(3), 514-516. doi: 10.1007/s10535-007-0109-6
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