biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:533-537, 2007 | DOI: 10.1007/s10535-007-0115-8

Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis

X. Yang1, X. Guo1, X. Zhang1,*, Y. Nie1, S. Jin1
1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, P.R. China

Protoplasts isolated from wild cotton Gossypium davidsonii were cultured in KM8P medium supplemented with different phytohormones. The most effective combination was 0.45 µM 2,4-dichlorophenoxyacetic acid, 2.68 µM α-naphthaleneacetic acid and 0.93 µM kinetin and the division percentage at the 8th day was 30.78 ± 3.04 %. The density of protoplasts at 2-10 × 105 cm-3 was suitable for protoplast division and calli formation, with a division percentage of 32.21 ± 3.64 % and a plating efficiency of 9.12 ± 2.61 % at the 40th day. The optimal osmotic potential was achieved using 0.5 M glucose or 0.1 M glucose plus 0.5 M mannitol. Protoplasts were cultured in three ways, a double-layer culture system, with liquid over solid medium was proved to be the best way. Embryo induction was further increased by addition of 0.14 µM gibberellic acid.

Keywords: cotton; 2,4-dichlorophenoxyacetic acid; gibberellic acid; kinetin; α-naphthaleneacetic acid; protoplast culture
Subjects: auxins; cotton; cytokinins; gibberellic acid, gibberellin; Gossypium davidsonii; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; protoplasts

Received: August 16, 2005; Accepted: June 20, 2006; Published: September 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Yang, X., Guo, X., Zhang, X., Nie, Y., & Jin, S. (2007). Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis. Biologia plantarum, 51(3), 533-537. doi: 10.1007/s10535-007-0115-8
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aydin, Y., Ipekci, Z., Talas-Ograş, T., Zehir, A., Bajrovic, K., Gozukirmizi, N.: High frequency somatic embryogenesis in cotton.-Biol. Plant. 48: 491-495, 2004. Go to original source...
    2. Bhojwani, S.S., Power, J.B., Cocking, E.C.: Isolation, culture and division of cotton callus protoplasts.-Plant Sci. Lett. 8: 85-89, 1977. Go to original source...
    3. Chen, Z.X., Li, S.J., Yue, J.X., Jiao, G.L., Liu, S.X., She, J.M., Wu, J.Y., Wang, H.B.: Plantlet regeneration from protoplasts isolated from an embryogenic suspension culture of cotton (Gossypium hirsutum L.).-Acta bot. sin. 31: 966-969, 1989.
    4. Fellner, M., Lebeda, A.: Callus induction and protoplast isolation from tissues of Cucumis sativus L. and C. melo L. seedlings.-Biol. Plant. 41: 11-24, 1998. Go to original source...
    5. Finer, J.J., Smith, R.H.: Isolation and culture of protoplasts from cotton (Gossypium klotzschianum Anderss) callus cultures.-Plant Sci. Lett. 26: 147-151, 1982. Go to original source...
    6. Firoozabady, E., DeBoer, D.L.: Isolation, culture and cell division in cotyledon protoplasts of cotton (Gossypium hirsutum and G. barbadense).-Plant Cell Rep. 5: 127-131, 1986. Go to original source...
    7. Frearson, E.M., Power, J.B., Cocking E.C.: The isolation, culture and regeneration of petunia leaf protoplasts.-Dev. Biol. 33: 130-137, 1973. Go to original source...
    8. Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension culture of soybean roots cells.-Exp. Cell Res. 50: 150-158, 1968. Go to original source...
    9. Hassanein, A.M, Soltan, D.M.: Solanum nigrum is a model system in plant tissue and protoplast cultures.-Biol. Plant. 43: 501-509, 2000. Go to original source...
    10. Jin, S., Zhang, X., Liang, S., Nie, Y., Guo, X., Huang, C.: Factors affecting transformation efficiency of embryogenic callus of upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens.-Plant Cell Tissue Organ Cult. 81: 229-237, 2005. Go to original source...
    11. Jumin, H.B., Nito, N.: Plant regeneration via somatic embryogenesis from protoplasts of six plant species related to Citrus.-Plant Cell Rep. 15: 332-336.1996. Go to original source...
    12. Kao, H.M., Seguin-Swartz, G.: Study of factors affecting the culture of Brassica napus L. and B. juncea Coss. mesophyll protoplasts.-Plant Cell Tissue Organ Cult. 10: 79-90, 1987. Go to original source...
    13. Kao, K.N., Michayluk, M.R.: Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media.-Planta 126: 105-110, 1975. Go to original source...
    14. Kyozuka, J., Hayashi, Y., Shimamoto, K.: High frequency plant regeneration from rice protoplasts by novel nurse culture methods.-Mol. gen. Genet. 206: 408-413, 1987. Go to original source...
    15. Lu, Z.X., Xia, Z.A.: Study on tissue and protoplast culture of Gossypium davidsonii.-Acta bot sin. 33: 98-103, 1991.
    16. Murashige, T., Skoog, F.: A revised medium for rapid grouth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473-479, 1962. Go to original source...
    17. Peeters, M.C., Willems, K., Swennen, R.: Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers.-Plant Cell Rep. 13: 208-211, 1994 Go to original source...
    18. Saka, K., Katterman, F.R., Thomas, J.C.: Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.).-Plant Cell Rep. 6: 470-472, 1987. Go to original source...
    19. Sun, Y., Zhang, X., Nie, Y., Guo, X., Jin S., Liang, S.: Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion.-Theor. appl. Genet. 109: 472-479, 2004. Go to original source...
    20. Sun, Y., Zhang, X., Huang, C., Nie, Y., Guo, X.: Plant regeneration via somatic embryogenesis from protoplasts of six explants in Coker 201 (Gossypium hirsutum L.).-Plant Cell Tissue Organ Cult. 82: 309-315, 2005. Go to original source...
    21. Vazquez-Tello, A., Hidaka, M., Uozumi, T.: Somatic embryogenesis and plant regeneration from isolated protoplasts of Lavatera thuringiaca.-Plant Cell Tissue Organ Cult. 40: 169-177, 1995. Go to original source...

    Return to the content