biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:538-540, 2007 | DOI: 10.1007/s10535-007-0116-7

A modified low copy number binary vector pUN for Agrobacterium-mediated plant transformation

E. Vaculková1, J. Moravčíková1, I. Matušíková1, M. Bauer2, J. Libantová1,*
1 Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic
2 Research Institute of Animal Production, Nitra, Slovak Republic

A modified low copy number plant binary vector pUN has been constructed and successfully used to clone unstable DNA sequences. The vector pUN comprises of low copy number, broad host range RK2 replicon from pBin19 and of multiple cloning site (MCS) and T-DNA region, both from a pBINPLUS-derived pLV06 vector. The absence of the ColE1 replicon in the backbone of the binary vector significantly contributed to stability of hardly clonable DNA sequences and enabled their transfer into the tobacco plants through Agrobacterium-mediated transformation.

Keywords: ColE1 replicon; Ti vector; unstable DNA sequences
Subjects: Agrobacterium tumefaciens; glucuronidase

Received: September 12, 2005; Accepted: March 2, 2006; Published: September 1, 2007  Show citation

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Vaculková, E., Moravčíková, J., Matušíková, I., Bauer, M., & Libantová, J. (2007). A modified low copy number binary vector pUN for Agrobacterium-mediated plant transformation. Biologia plantarum, 51(3), 538-540. doi: 10.1007/s10535-007-0116-7
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