biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:579-583, 2007 | DOI: 10.1007/s10535-007-0127-4

Cytokinin oxidase/dehydrogenase activity as a tool in gibberellic acid/cytokinin cross talk

D. Todorova1, I. Vaseva1, J. Malbeck2, A. Trávníčková2, I. Macháčková2,*, E. Karanov1
1 Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

Changes in endogenous cytokinin (CK) content and cytokinin oxidase/dehydrogenase activity (CKX) in response to gibberellic acid (GA3) in two pea cultivars with different life span were assessed. The control leaves of cv. Scinado, which developed faster, had higher initial cytokinin content and lower CKX activity, while opposite trend was observed in cv. Manuela with longer life span. Increased CKX and decreased CK content were detected in leaves of cv. Scinado after treatments with 0.5, 1 and 5 µM GA3. Changes in CK content and CKX activity in GA3-treated cv. Manuela leaves were reciprocal to those in cv. Scinado. CK content and CKX activity in roots were not significantly influenced by the application of GA3. The slight repression of CKX activity in some of the root samples was accompanied by increased isopentenyladenine and isopentenyladenine riboside content. Obtained results suggest that CKX was responsible for the changes in endogenous cytokinin pool in GA3-treated plants and most probably this enzyme represents an important link in GA/cytokinin cross talk.

Keywords: isopentenyladenine; pea; Pisum sativum; zeatin
Subjects: abscisic acid (ABA); cytokinin oxidase/dehydrogenase; gibberellic acid, gibberellin; isopentenyladenine; pea; Pisum sativum

Received: April 10, 2006; Accepted: September 20, 2006; Published: September 1, 2007  Show citation

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Todorova, D., Vaseva, I., Malbeck, J., Trávníčková, A., Macháčková, I., & Karanov, E. (2007). Cytokinin oxidase/dehydrogenase activity as a tool in gibberellic acid/cytokinin cross talk. Biologia plantarum, 51(3), 579-583. doi: 10.1007/s10535-007-0127-4
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