biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 51:647-652, 2007 | DOI: 10.1007/s10535-007-0137-2

Paclitaxel and baccatin III production induced by methyl jasmonate in free and immobilized cells of Taxus baccata

M. Bonfill1, S. Bentebibel1, E. Moyano2, J. Palazón1, R. M. Cusidó1,*, R. Eibl3, M. T. Piñol1
1 Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain
2 Departament de Ciències Experimentals i de Salut, Universitat Pompeu Fabra, Barcelona, Spain
3 Hochschule Wädenswill, Wädenswill, Switzerland

The effects of 100 and 200 µM methyl jasmonate (MJA) on cell proliferation and paclitaxel and baccatin III production were investigated in free and alginate immobilized cells of Taxus baccata growing in a selected product formation culture medium. The greatest accumulation of paclitaxel (13.20 mg dm-3) and baccatin III (4.62 mg dm-3) occurred when 100 µM MJA was added to the culture medium of cells entrapped using a 1.5 and 2.5 % alginate solution. The effects of different treatments on the viability of cultured cells and their capacity to excrete both taxanes into the surrounding medium were considered.

Keywords: alginate entrapped cells; cell cultures; elicitation; taxanes; yew
Subjects: auxins; baccatin; cell wall; cytokinins; immobilized cells; in vitro culture, cell suspension cultures; methyl jasmonate; nutrient medium, Gamborg (G5); paclitaxel; taxanes; Taxus baccata; yew

Received: August 16, 2005; Accepted: July 20, 2006; Published: December 1, 2007  Show citation

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Bonfill, M., Bentebibel, S., Moyano, E., Palazón, J., Cusidó, R.M., Eibl, R., & Piñol, M.T. (2007). Paclitaxel and baccatin III production induced by methyl jasmonate in free and immobilized cells of Taxus baccata. Biologia plantarum, 51(4), 647-652. doi: 10.1007/s10535-007-0137-2
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