biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:690-698, 2007 | DOI: 10.1007/s10535-007-0144-3

Ultrastructural alterations in mesophyll and bundle sheath chloroplasts of two maize cultivars in response to chilling at high irradiance

A. S. Saropulos1,*, D. S. H. Drennan1
1 Department of Agricultural Botany, the University of Reading, Reading, UK

Maize (Zea mays L.) seedlings of two cultivars (cv. Bastion adapted to W. Europe, and cv. Batan 8686 adapted to the highlands of Mexico), raised in a glasshouse (19-25 °C), were transferred to 4.5 or 9 °C at photon flux density (PPFD) of 950 µmol m-2 s-1 with 10-h photoperiod for 58 h and then allowed to recover at 22 °C for 16 h (14 h dark and 2 h at PPFD of 180 µmol m-2 s-1). The ultrastructural responses after 4 h or 26 h at 4.5 °C were the disappearance of starch grains in the bundle sheath chloroplasts and the contraction of intrathylakoid spaces in stromal thylakoids of the mesophyll chloroplasts. At this time, bundle sheath chloroplasts of cv. Batan 8686 formed peripheral reticulum. Prolonged stress at 4.5 °C (50 h) caused plastid swelling and the dilation of intrathylakoid spaces, mainly in mesophyll chloroplasts. Bundle sheath chloroplasts of cv. Batan 8686 seedlings appeared well preserved in shape and structure. Batan 8686 had also higher net photosynthetic rates during chilling and recovery than Bastion. Extended leaf photobleaching developed during the recovery period after chilling at 4.5 °C. This was associated with collapsed chloroplast envelopes, disintegrated chloroplasts and very poor staining.

Keywords: low temperature; photooxidation; photosynthesis; Zea mays
Subjects: bundle sheath; chloroplast, chilling; chloroplast ultrastructure; cultivar and genotype differences; gas exchange; maize; photon flux density (PPFD, PFD); Zea mays

Received: November 23, 2005; Accepted: August 8, 2006; Published: December 1, 2007  Show citation

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Saropulos, A.S., & Drennan, D.S.H. (2007). Ultrastructural alterations in mesophyll and bundle sheath chloroplasts of two maize cultivars in response to chilling at high irradiance. Biologia plantarum, 51(4), 690-698. doi: 10.1007/s10535-007-0144-3
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