biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:699-706, 2007 | DOI: 10.1007/s10535-007-0145-2

UV-B response of green and etiolated barley seedlings

I. Fedina1,*, M. Velitchkova2, K. Georgieva1, K. Demirevska1, L. Simova1
1 Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria

7-d-old etiolated and green barley seedlings (Hordeum vulgare L. cv. Alfa) were irradiated with UV-B for 30 min and then kept for 24 h in light or darkness. Chlorophyll (Chl) synthesis was inhibited by about 30 % as a result of UV-B irradiation, but there were no significant changes in photochemical activity measured by variable to maximum fluorescence ratio (Fv/Fm), quantum yield (ΦPS2) and oxygen evolution rate. Electron transport of etiolated seedlings was similar to that of green ones, nevertheless, the Chl content was more then 2-fold lower. Ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits were diminished as a result of UV-B irradiation in etiolated and green plants, especially in those kept in the darkness. Catalase activity decreased and total superoxide dismutase activity increased in green and etiolated plants following UV-B treatment. When benzidine was used as a substrate, an isoform located between guaiacol peroxidases 2 and 3 (guaiacol peroxidase X) appeared, which was specific for UV-B treatment. As a result of irradiation, the contents of UV-B absorbing and UV-B induced compounds increased in green seedlings but not in etiolated seedlings.

Keywords: chlorophyll fluorescence; flavonoids; Hordeum vulgare; oxygen evolution; ribulose-1,5-bisphosphate carboxylase, oxygenase
Subjects: barley; carotenoids; catalase; chlorophyll fluorescence; flavonoids; Hordeum vulgare; malondialdehyde; oxygen evolution; photon flux density (PPFD, PFD); photosystems 1,2; proline; proteins; quaicol peroxidase; ribulose-1,5-bisphosphate carboxylase/oxygenase; superoxide dismutase (SOD); UV radiation, stress

Received: January 16, 2006; Accepted: August 8, 2006; Published: December 1, 2007  Show citation

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Fedina, I., Velitchkova, M., Georgieva, K., Demirevska, K., & Simova, L. (2007). UV-B response of green and etiolated barley seedlings. Biologia plantarum, 51(4), 699-706. doi: 10.1007/s10535-007-0145-2
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    References

    1. Aebi, H.: Catalase in vitro.-Methods Enzymol. 105: 121-126, 1984. Go to original source...
    2. Bates, L.S., Waldren, R.O., Teare, I.D.: Rapid determination of free proline for water-stress studies.-Plant Soil 39: 205-207, 1973. Go to original source...
    3. Beauchamp, C., Fridovich, Y.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.-Anal. Biochem. 44: 276-287, 1971. Go to original source...
    4. Beggs, C.J., Wellmann, E.: Photocontrol of flavonoid biosynthesis.-In: Kedrick, R.E., Kronenberg, G.H.M. (ed.): Photomorphogenesis in Plants. Pp. 733-751. Kluwer Academic Publishers, Dordrecht 1994. Go to original source...
    5. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    6. Britt, A.B.: DNA damage and repair in higher plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 75-100, 1996. Go to original source...
    7. Cen, Y.P., Bornman, J.P.: The effect of exposure to enhanced UV-B radiation on the penetration of monochromatic and polychromatic UV-B radiation in leaves of Brassica napus.-Physiol. Plant. 87: 249-255, 1993. Go to original source...
    8. Demirevska-Kepova, K., Simova-Stoilova, L., Stoyanova, Z., Hölzer, R., Feller, U.: Biochemical changes in barley plants after excessive supply of copper and manganese.-Environ. exp. Bot. 52: 253-266, 2004. Go to original source...
    9. Dunlap, W.C., Yamamoto, T.: Small molecule antioxidants in marine organisms: antioxidant activity of mycosporine-glycine.-Comp. Biochem. Physiol. B 112: 105-114, 1995. Go to original source...
    10. Esterbauer, H., Cheeseman, K.: Determination of aldehyde lipid peroxidation products: malonaldehyde and hydroxynonenal.-Methods Enzymol. 186: 407-431, 1990. Go to original source...
    11. Fedina, I., Grigorova, I., Georgieva, K.: Response of barley seedlings to UV-B radiation as affected by NaCl.-J. Plant Physiol. 160: 205-208, 2003. Go to original source...
    12. Fedina, I., Velitchkova, M., Georgieva, K., Grigorova, I.: UV-B-induced compounds as affected by proline and NaCl in Hordeum vulgare L. cv. Alfa.-Environ. exp. Bot. 54:182-191, 2005. Go to original source...
    13. Foyer, C.R., Lelandais, M., Kunert, K.: Photooxidative stress in plants.-Physiol. Plant. 92: 696-717, 1994. Go to original source...
    14. Gerhardt, K.E., Wilson, I., Greenberg, B.M.: Tryptophan photolysis leads to a UVB-induced 66 kDa photoproduct of ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco) in vitro and in vivo.-Photochem. Photobiol. 70: 49-56, 1999. Go to original source...
    15. González, A., Steffen, K.L., Lynch, J.: Light and excess manganese. Implications for oxidative stress in common bean.-Plant Physiol. 118: 493-504, 1998. Go to original source...
    16. Gonzales, R., Paul, N.D., Perey K., Ambrose, P.K., McLaughlin, C.K., Barnes, J.D., Areses, M., Wellburn, A.R.: Responses to ultraviolet-B radiation (280-315 nm) of pea (Pisum sativum) lines differing in leaf surface wax.-Physiol. Plant. 98: 852-860, 1996. Go to original source...
    17. Hahlbrock, K., Scheel, D.: Physiology and molecular biology of phenylpropanoid metabolism.-Annu. Rev. Plant Physiol. Plant mol. Biol. 40: 347-369, 1989. Go to original source...
    18. Hart, M.A., Tyson, H., Bloomberg, B.: Measurement of activity of peroxidase isoenzymes in flax.-Can. J. Bot. 49: 2129-2137, 1971. Go to original source...
    19. Jordan, B.R., James, P.E., Strid, A., Anthony, R.G.: The effect of ultraviolet-B radiation on gene expression and pigment composition in etiolated and green pea leaf tissue: UV-B-induced changes are gene-specific and dependent upon the developmental stage.-Plant Cell Environ. 17: 45-54, 1994. Go to original source...
    20. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    21. Lichtenthaler, H.K.: Chlorophylls and carotenoids-pigments of photosynthetic biomembranes.-Methods Enzymol. 148: 350-382, 1987. Go to original source...
    22. Mackerness, S.A.H., Butt, J.P., Jordan, B.R.: Amelioration of ultraviolet-B-induced down regulation of mRNA transcript for chloroplast proteins, by irradiance, is mediated by photosynthesis.-J. Plant Physiol. 148: 100-106, 1996. Go to original source...
    23. Malanga, R., Kozak, R., Puntarulo, S.: N-Acetylcysteine-dependent protection against UV-B damage in two photosynthetic organisms.-Plant Sci. 41: 129-137, 1999. Go to original source...
    24. Markham, K.R., Ryan, K.G., Bloor, S.G., Mitchell, K.A.: An increase in luteolin:apigenin ratio in Marchantia polymorpha on UV-B enhancement.-Phytochemistry 48: 791-794, 1998. Go to original source...
    25. McRae, D.G., Thompson, J.E.: Senescence-dependent changes in superoxide anion production by illuminated chloroplasts from bean leaves.-Planta 158: 185-193, 1983. Go to original source...
    26. Mirecki, R.M., Teramura, A.H.: Effects of ultraviolet-B irradiance on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion.-Plant Physiol. 74: 475-480, 1984. Go to original source...
    27. Murali, N.S., Teramura, A.N.: Intraspecific difference in Cucumis sativus sensitivity to ultraviolet-B radiation.-Physiol. Plant. 68: 673-677, 1986. Go to original source...
    28. Prasad, S.M., Zeeshan, M.: UV-B radiation and cadmium induced changes in growth, photosynthesis, and antioxidant enzymes of cyanobacterium Plectonema boryanum.-Biol. Plant. 49: 229-236, 2005. Go to original source...
    29. Rao, M.V., Paliyathy, G., Ormrod, D.P.: Ultraviolet-B and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.-Plant Physiol. 110: 125-136, 1996. Go to original source...
    30. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986. Go to original source...
    31. Sharma, P.K., Anand, P., Sankhalkar, S.: Oxidative damage and changes in activities of antioxidant enzymes in wheat seedlings exposed to ultraviolet-B radiation.-Curr. Sci. 75: 359-366, 1998.
    32. Smith, J.L., Burritt, D.J., Bannister, P.: Shoot dry weight, chlorophyll and UV-B-absorbing compounds as indicator of a plant's sensivity to UV-B radiation.-Ann. Bot. 86: 1057-1063, 2000. Go to original source...
    33. Strid, A.: Alteration in expression of defence genes in Pisum sativum after exposure to supplementary ultraviolet-B radiation.-Plant Cell Physiol. 34: 949-953, 1993.
    34. Tekchandani, K, Guruprasad, N.: Modulation of guaiacol peroxidase inhibitor by UV-B in cucumber cotyledons.-Plant Sci. 136: 131-137, 1998. Go to original source...
    35. Tullberg, A., Alexciev, K., Pfannschmidt, T., Allen, J.F.: Photosynthetic electron flow regulates transcription of the psaB gene in pea (Pisum sativum L.) chloroplasts through the redox state of the plastoquinine pool.-Plant Cell Physiol. 41: 1045-1054, 2000. Go to original source...
    36. Tyystjarvi, E., Karunen, J.: A microcomputer program and fast analog to digital converter card for the analysis of fluorescence induction transients.-Photosynth. Res. 26: 127-132, 1990. Go to original source...
    37. Van Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology.-Photosynth. Res. 25: 147-150, 1990. Go to original source...
    38. Woodbury, W., Spencer, A.K., Stahmann, M.A.: An improved procedure using ferricyanide for detecting catalase isozymes.-Anal. Biochem. 44: 301-305, 1971. Go to original source...

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