biologia plantarum

Biologia plantarum 51:746-748, 2007 | DOI: 10.1007/s10535-007-0152-3

Transgenic tobacco plants containing Bt and GNA genes

H. Y. Zhang^1,2,*, X. Z. Liu¹, L. Wei³, L. Y. Zhou³, Y. M. Yang¹

¹ Southwest Forestry College, White Dragon Temple, Kunming, Yunnan Province, P.R. China

² South China University of Tropic Agriculture, Dangzhao, Hainan, P.R.China

³ Institute of Microbiology, Chinese Academy of Science, Beijing, P.R. China

A new plant expression vector (pBSbtCry1Ac-GNA) containing two insect resistant genes, a synthetic chimeric gene SbtCry1Ac encoding the insecticidal protein CrylAc and a gene GNA encoding snowdrop lectin (Galanthus nivalis agglutinin) was constructed. Transgenic tobacco plants containing these two genes were obtained through Agrobacterium-mediated transformation of tobacco leaf discs. Results from PCR detection and genomic DNA Southern blot analysis indicated that both SbtCrylAc gene and GNA gene were integrated into the genome of these plants. Results of Western blot analysis indicated that these two proteins were expressed in the analyzed plants. Bioassays of Myzus persicae and Helicoverpa assulta on detached leaves of transformed tobacco plants were carried out. The average aphid inhibition rate of these plants tested at 12 d post-infestation was 71.9 %. The average H. assulta mortality of these plants tested at 6 d post-infestation was up to 89.8 %. The kanamycin resistance of the T₁ progeny of these transgenic plants was analyzed and a typical 3:1 segregation was observed.

Keywords: agglutinin; insect-resistant; SbtcrylAc

Subjects: nutrient medium, Murashige and Skoog (MS); polymerase chain reaction (PCR); tobacco

Received: October 15, 2004; Accepted: September 10, 2006; Published: December 1, 2007  Show citation