biologia plantarum

Biologia plantarum 51:749-753, 2007 | DOI: 10.1007/s10535-007-0153-2

Proliferation and differentiation from endosperms of Carthamus tinctorius

N. Walia¹, A. Kaur¹, S. B. Babbar^1,*

¹ Department of Botany, University of Delhi, Delhi, India

The endosperms of Carthamus tinctorius cv. HUS-305, excised at globular to heart-shaped stages of zygotic embryo development, were cultured on Murashige and Skoog's medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthalene-acetic acid (NAA). The highest incidence of callusing was on 2,4-D supplemented media. However, embryos differentiated only from the calli developed on media supplemented with BAP, kinetin or TDZ with the last eliciting maximum embryogenic response. The addition of a reduced nitrogen source, casein hydrolysate to MS medium supplemented with BAP and/or NAA, did not stimulate the response. However, adenine sulphate (100 mg dm^-3) promoted the induction of somatic embryos. Upon transfer to MS basal medium or the same supplemented with 0.61 µM gibberellic acid (GA₃), plumular poles of few embryos elongated resulting in the development of shoots.

Keywords: Asteraceae; endosperm culture; growth regulators; safflower; triploids

Subjects: abscisic acid (ABA); auxins; Carthamus tinctorius; cytokinins; endosperm; gibberellic acid, gibberellin; ploidy; safflower; sufflower; thidiazuron

Received: August 22, 2005; Accepted: July 25, 2006; Published: December 1, 2007  Show citation