biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:759-763, 2007 | DOI: 10.1007/s10535-007-0155-0

Plasma membrane ultrastructure in embryogenic cultures of orchardgrass during NaCl stress

S. Dutta Gupta1,*
1 Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur, India

Embryogenic cultures of Dactylis glomerata L. were subjected to NaCl stress by culturing for 4 passages in Schenk and Hildebrandt (SH) medium containing 30 µM dicamba and 200 mM NaCl. Ultrastructural studies indicated invaginations and disruptions of the plasma membrane. Membrane-bound vesicles were observed in the cytoplasm of NaCl treated cells and their occurrence were increased with the culture age.

Keywords: Dactylis glomerata L; in vitro cells; membrane damage; plasmolysis; salt stress; vesiculation
Subjects: Dactylis glomerata; in vitro culture, embryogenesis, somatic embryogenesis; nutrient medium, Schenk and Hildebrandt (SH); orchardgrass; plasma membrane; plasmolysis; salinity, salt stress

Received: August 22, 2005; Accepted: July 15, 2006; Published: December 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Dutta Gupta, S. (2007). Plasma membrane ultrastructure in embryogenic cultures of orchardgrass during NaCl stress. Biologia plantarum, 51(4), 759-763. doi: 10.1007/s10535-007-0155-0
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Auge, R.M., Stodola, Ann J.W., Dutta Gupta, S., Hovanesian, J.C., Conger, B.V.: Using pressure-volume analysis to characterize NaCl-induced osmotic and turgor adjustment in in vitro cultures.-J. Plant Physiol. 136: 15-23, 1990. Go to original source...
    2. Battey, N.H., James, N.C., Greenland, A.J., Brownlee, C.: Exocytosis and endocytosis.-Plant Cell 11: 643-660, 1999. Go to original source...
    3. Blumwald, E., Gelli, A.: Secondary inorganic ion transport at the tonoplast.-Adv. Bot. Res. 25: 401-407, 1997. Go to original source...
    4. Bonghanmi, N., Michonneau, P., Verdus, M.C., Piton, F., Ferjani, E., Bizid, E., Fleurat-Lessard, P.: Structural changes induced by NaCl in companion and transfer cells of Medicago sativa blades.-Protoplasma 220: 179-187, 2003. Go to original source...
    5. Cherian, S., Reddy, M.P.: Evaluating of NaCl tolerance in the callus cultures of Suaeda nudiflora Moq.-Biol. Plant. 46: 193-198, 2003. Go to original source...
    6. Ciamporova, M., Mistrik, I.: The ultrastructural response of root cells to stressful conditions.-Environ. exp. Bot. 33: 11-26, 1993. Go to original source...
    7. Conger, B.V., Hanning, G.E.: Registration of Embryogen-P orchardgrass germplasm with a high capacity for somatic embryogenesis from in vitro cultures.-Crop Sci. 31: 855, 1991. Go to original source...
    8. Diaz de Leon, J.L., Soto, H., Merchant, M.T., Diaz de Leon, L. Biochemical and ultrastructural changes induced by NaCl in cell cultures derived from Bouvardia ternifolia.-In: San Pietro, A. (ed.): Biosaline Research. Pp. 461-466. Plenum Press, New York 1982. Go to original source...
    9. Dowgert, M.F., Steponkus, P.L.: Behavior of the plasma membrane of isolated protoplast during the freeze-thaw cycle.-Plant Physiol. 75: 1139-1151, 1984. Go to original source...
    10. Dutta Gupta, S.: Protein profiles of somatic embryos and regenerated plants from NaCl selected and control cultures of orchardgrass.-Biol. Plant. 42: 297-302, 1999. Go to original source...
    11. Dutta Gupta, S., Auge, R.M., Denchev, P.D., Conger, B.V.: Growth, proline accumulation and water relations of NaCl-selected and non-selected callus lines of Dactylis glomerata L.-Environ. exp. Bot. 35: 83-92, 1995. Go to original source...
    12. Dutta Gupta, S., Joshi, P., Hovanesian, J.C., Conger, B.V.: Ultrastructural characterization of somatic embryos regenerated from NaCl selected and non-selected calli of Dactylis glomerata L.-Protoplasma 170: 177-185, 1992. Go to original source...
    13. Echeverria, E.: Vesicle-mediated solute transport between the vacuole and the plasma membrane.-Plant Physiol. 123: 1217-1226, 2000. Go to original source...
    14. Elavumoottil, O.C., Martin, J.P., Moreno, M.L.: Changes in sugars, sucrose synthase activity and proteins in salinity tolerant callus and cell suspension cultures of Brassica oleracea L.-Biol. Plant. 46: 7-12, 2003. Go to original source...
    15. Gananapragasam, S., Vasil, I.K.: Ultrastructural changes in suspension culture cells of Panicum maxum during cryopreservation.-Plant Cell Rep. 11: 169-174, 1992. Go to original source...
    16. Gordon-Kamm, W.J., Steponkus, P.L.: The behavior of plasma membrane following osmotic contraction of isolated protoplasts: Implications in freezing injury.-Protoplasma 123: 83-94, 1984. Go to original source...
    17. Hasegawa, P.M., Bressan, R.A., Zhu, J.K., Bohnert, H.J.: Plant cellular and molecular responses to high salinity.-Ann. Rev. Plant Physiol. Plant mol. Biol. 51: 463-499, 2000. Go to original source...
    18. Leopold, A.C., Willing, R.P.: Evidence for toxicity effects of salt on membranes.-In: Staples, R.C., Toenniessen, G.H. (ed): Salinity Tolerance in Plants, Strategies for Crop Improvement. Pp. 67-76. Wiley, New York 1984.
    19. Lerner, H.R.: Adaptation to salinity at the plant cell leveL.-Plant Soil 89: 3-14, 1985. Go to original source...
    20. Mitsuya, S., Takeoka, Y., Miyake, H.: Effects of sodium chloride on foliar ultrastructure of sweet potato (Ipomoea batatas Lam.) plantlets grown under light and dark conditions in vitro.-J. Plant Physiol. 57: 661-667, 2000. Go to original source...
    21. Molassiotis, A.N., Sotiropoulos, T., Tanou, G., Kofidis, G., Diamantidis, G., Therios, I.: Antioxidant and anatomical responses in shoot culture of the apple rootstock MM 106 treated with NaCl, KCL, mannitol or sorbitol.-Biol. Plant. 50: 61-68, 2006. Go to original source...
    22. Niknam, V., Bagherzadeh, M., Ebrahimzadeh, H., Sokhansanj, A.: Effect of NaCl on biomass and contents of sugars, proline and proteins in seedlings and leaf explants of Nicotiana tabacum grown in vitro.-Biol. Plant. 48: 613-615, 2004. Go to original source...
    23. Niu, X., Damsz, B., Kononowicz, A.K., Bressan, R.A., Hasegawa, P.M.: NaCl-induced alterations in both cell structure and tissue specific plasma membrane H+-ATPase gene expression.-Plant Physiol. 111: 679-686, 1996. Go to original source...
    24. Nyman, L.P., Walter, R.J., Donovan, R.D., Berns, M.W., Arditti, J.: Effects of artificial sea water on the ultrastructure and morphometry of taro (Colocasia esculenta, Areceae) cells in vitro.-Environ. exp. Bot. 27: 245-252, 1987. Go to original source...
    25. Palta, J.P.: Stress interactions at the cellular and membrane levels.-Hort. Sci. 25: 1377-1381, 1990. Go to original source...
    26. Panda, S.K., Upadhyay, R.K.: Salt stress injury induces oxidative alterations and antioxidative defence in the roots of Lemna minor.-Biol. Plant. 48: 249-253, 2004. Go to original source...
    27. Pareek, A., Singla, S.L., Grover, A.: Short-term salinity and high temperature stress-associated ultrastructural alterations in young leaf cells of Oryza sativa L.-Ann. Bot. 80: 629-639, 1997. Go to original source...
    28. Parida, A.K., Das, A.B., Mittra, B.: Effects of NaCl stress on the structure, pigment complex composition, and photosynthetic activity of mangrove Bruguiera parviflora chloroplast.-Photosynthetica 41: 191-200, 2003. Go to original source...
    29. Schenk, R.U., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199-204, 1972. Go to original source...
    30. Shimony, C., Fahn, A., Reinhold, L.: Ultrastructure and ion gradients in the salt glands of Avicenia marina.-New Phytol. 72: 27-36, 1973. Go to original source...
    31. Spurr, A.R.: A low viscosity epoxy resin embedding medium for electron microscopy.-J. Ultrastructural Res. 26: 31-43, 1969. Go to original source...
    32. Steponkus, P.L., Wolfe, J., Dowgert, M.F.: Stress induced by contraction and expansion during a freeze-thaw cycle.-In: Morris, G.J., Clarke, A. (ed.): Effects of Low Temperatures on Biological Membranes. Pp. 307-322. Academic Press, London 1981.
    33. Steponkus, P.L.: Fundamental aspects of cryoinjury as related to cryopreservation of plant cells and organs.-In: Zaitlin, M., Day, P., Hollander, A. (ed.): Biotechnology in Plant Science. Pp. 145-159. Academic Press, New York 1985. Go to original source...
    34. Wolfe, J., Steponkus, P.L.: The stress-strain relation of the plasma membrane of isolated plant protoplasts.-Biochem. biophys. Acta 643: 663-668, 1981. Go to original source...
    35. Yamane, K., Kawasaki, M., Taniguchi, M., Miyaki, H.: Differential effects of NaCl and polyethylene glycol on the ultrastructure of chloroplasts in rice seedlings.-J. Plant Physiol. 160: 573-575, 2003. Go to original source...
    36. Zhu, J.K.: Plant salt tolerance.-Trends Plant Sci. 6: 66-71, 2001. Go to original source...

    Return to the content