biologia plantarum

Biologia plantarum 51:773-776, 2007 | DOI: 10.1007/s10535-007-0158-x

The effect of sugars on niger embryogenesis and plant regeneration in anther culture

B. P. Hema¹, H. N. Murthy^1,*

¹ Department of Botany, Karnatak University, Dharwad, India

The influence of different sugars (sucrose, maltose, glucose and fructose, 0.05-0.5 M) on embryogenesis and plant regeneration from cultured anthers of niger [Guizotia abyssinica (L. f.) Cass.] have been studied. Among the different sugars tested, 0.2 M sucrose was the best for embryo induction and plant regeneration. Maximum of 57 embryos per 60 anthers were induced on embryo induction medium [Gamborg's B5 medium supplemented with 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 2 µM kinetin (KIN)] containing 0.2 M sucrose. Embryo differentiation was achieved on B5 medium supplemented with 0.5 µM benzyladenine (BA) and 0.09 M sucrose. Embryo maturation was on B5 medium containing 10 µM abscisic acid (ABA) and 0.09 M sucrose. Embryo germination was achieved on B5 medium with 0.09 M sucrose. Embryos that were developed on B5 medium supplemented with 0.2 M sucrose showed highest frequency (68 %) of plant regeneration.

Keywords: fructose; glucose; growth regulators; Guizotia abyssinica; haploids; maltose; sucrose

Subjects: abscisic acid (ABA); anther culture; cytokinins; Guizotia abyssinica; in vitro culture, callus growth and duration; niger; nutrient medium, Gamborg (B5); sugars

Received: November 18, 2005; Accepted: July 23, 2006; Published: December 1, 2007  Show citation