biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:782-786, 2007 | DOI: 10.1007/s10535-007-0160-3

In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds

M. Sujatha1,*, V. Dinesh Kumar1
1 Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, Hyderabad, India

The organogenic competence of leaf explants of eleven Carthamus species including C. tinctorius on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) + α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) + NAA was investigated. Highly prolific adventitious shoot regeneration was observed in C. tinctorius and C. arborescens on both growth regulator combinations and the shoot regeneration frequency was higher on medium supplemented with TDZ + NAA. Nodal culture of nine Carthamus species on media supplemented with BA and kinetin (KIN) individually revealed the superiority of media supplemented with BA over that of KIN in facilitating a higher shoot proliferation index. Proliferating shoots from axillary buds and leaf explants were transferred to medium supplemented with 1.0 mg dm-3 KIN or 0.5 mg dm-3 BA for shoot elongation. Elongated shoots were rooted on half-strength MS medium supplemented with 1.0 mg dm-3 each of indole-butyric acid (IBA) and phloroglucinol. The plantlets thus obtained were hardened and transferred to soil.

Keywords: direct organogenesis; growth regulators; in vitro culture; tissue culture; wild safflowers
Subjects: auxins; bud in vitro regeneration; Carthamus arborescens; Carthamus tinctorius; cytokinins; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog (MS); safflower; sufflower; thidiazuron

Received: November 28, 2005; Accepted: July 25, 2006; Published: December 1, 2007  Show citation

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Sujatha, M., & Dinesh Kumar, V. (2007). In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds. Biologia plantarum, 51(4), 782-786. doi: 10.1007/s10535-007-0160-3
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