biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:66-71, 2008 | DOI: 10.1007/s10535-008-0009-4

Induction of alternative oxidase chain under salt stress conditions

A. L. Ferreira1,*, J. D. Arrabaça2, V. Vaz-Pinto2, M. E. Lima-Costa1
1 Faculdade de Engenharia de Recursos Naturais, Universidade do Algarve, Campus de Gambelas, Gambelas, Portugal
2 Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal

This paper describes the effect of NaCl on the respiration of Citrus cell suspensions namely on the induction of the alternative oxidase. The exposure of two Citrus (cvs. Carvalhal tangor and Valencia late) cell suspensions to 200 or 400 mM NaCl lead to a reduction on cell respiration rates. Under these conditions, the respiration rate decreased less in the presence of KCN indicating a stimulation of the capacity of the alternative oxidase (AOX). In addition, immunoblots showed an increase on the amount of AOX protein. Antibodies raised against the Sauromatum guttatum enzyme recognized the reduced form of the enzyme near the 35 kDa band. The protein accumulation was correlated with the significantly higher AOX capacity observed for cv. Carvalhal tangor.

Keywords: cell suspension; Citrus; respiration rate
Subjects: alternative oxidase chain; Citrus; growth analysis, biomass and yield enhancement; in vitro culture, cell suspension; nutrient medium, Murashige and Skoog (MS); proteins; reactive oxygen species (ROS); respiration; salicylic acid; salinity, salt stress; sugars

Received: April 27, 2006; Accepted: October 23, 2006; Published: March 1, 2008  Show citation

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Ferreira, A.L., Arrabaça, J.D., Vaz-Pinto, V., & Lima-Costa, M.E. (2008). Induction of alternative oxidase chain under salt stress conditions. Biologia plantarum, 52(1), 66-71. doi: 10.1007/s10535-008-0009-4
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    References

    1. Amthor, J.S.: The McCree-de Wit-Penning de Vries-Thornley respiration paradigms: 30 years later.-Ann. Bot. 86: 1-20, 2000. Go to original source...
    2. Aubert, S., Gout, E., Bligny, R., Douce R.: Induction of alternative oxidase synthesis by herbicides inhibiting branched-chain amino acid synthesis.-Plant J. 11: 649-657, 1997. Go to original source...
    3. Bartoli, C.G., Gomez, F., Gergoff, G., Guiamet, J.J., Puntarulo, S.: Up-regulation of the mitochondrial alternative oxidase enhances photosynthetic electron transport under drought conditions.-J. exp. Bot. 56: 1269-1276, 2005. Go to original source...
    4. Duque P., Arrabaça J.D.: Respiratory metabolism during cold storage of apple fruit. II. Alternative oxidase is induced at the climacteric.-Physiol. Plant. 107: 24-31, 1999. Go to original source...
    5. Elthon, T.E., Nickels, R.L., McIntosh, L.: Monoclonal antibodies to the alternative oxidase of higher plant mitochondria.-Plant Physiol. 89: 1311-1317, 1989. Go to original source...
    6. Ferreira, A.L., Lima-Costa, M.E.: Metabolic responses to salt stress in cell suspension cultures of sensitive and resistant Citrus.-JHSB 81: 983-988, 2006. Go to original source...
    7. Fung, R.W.M., Wang, C.Y., Smith, D.L., Gross, K.C., Tian, M.: MeSA and MeJA increase steady-state transcript levels of alternative oxidase and resistance against chilling injury in sweet peppers (Capsicum annuum L.).-Plant Sci. 166: 711-719, 2004. Go to original source...
    8. Geraldes-Laakso, S., Arrabaça, J.D.: Respiration and heat production by soybean hypocotyl and cotyledon mitochondria.-Plant Physiol. Biochem. 35: 897-903, 1997.
    9. González-Meler, M.A., Giles L, Thomas, R.B., Siedow, J.M.: Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply.-Plant Cell Environ. 24: 205-215, 2001 Go to original source...
    10. Hiser, C., McIntosh, L.: Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices.-Plant Physiol. 93: 312-318, 1990 Go to original source...
    11. Jebara, M., Harzalli-Jebara, S., Payré, H., Aouani, M.E., Drevon, J.J.: Influence of salinity and abscisic acid on the O2 uptake by N2-fixing nodules of common bean.-Biol. Plant. 50: 717-721, 2006. Go to original source...
    12. Kearns, A., Whelan, J., Young, S., Elthon, T.E., Day, D.A.: Tissue-specific expression of the alternative oxidase in soybean and siratro.-Plant Physiol. 99: 712-717, 1992. Go to original source...
    13. Kirimura, K., Matsui, T., Sugano, S., Usami, S.: Enhancement and repression of cyanide-insensitive respiration in Aspergillus ringer.-FEMS Microbiol. Lett. 141: 251-254, 1996. Go to original source...
    14. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-686, 1970. Go to original source...
    15. Lennon, A.M., Neuenschwander, U.H., Ribas-Carbo, M., Giles, L., Ryals, J.A., Siedow, J.N.: The effects of salicylic acid and tobacco mosaic virus infection on the alternative oxidase of tobacco.-Plant Physiol. 115: 783-791, 1997. Go to original source...
    16. Maxwell, D.P., Wang, Y., McIntosh, L.: The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells.-Proc. nat. Acad. Sci. USA 96: 8271-8276, 1999. Go to original source...
    17. Meeuse, B.J.D.: Thermogenic respiration in aroids.-Annu. Rev. Plant Physiol. 26: 117-126, 1975. Go to original source...
    18. Millar, A.H., Wiskich, J.T., Whelan, J., Day, D.A.: Organic acid activation of the alternative oxidase of plant mitochondria.-FEBS Lett. 329: 259-262, 1993. Go to original source...
    19. Mittler, R.: Oxidative stress, antioxidants and stress tolerance.-Trends Plant Sci. 7: 405-410, 2002. Go to original source...
    20. Mizuno, M., Tada, Y., Uchii, K., Kawakami, S., Mayama, S.: Catalase and alternative oxidase cooperatively regulate programmed cell death induced by β-glucan elicitor in potato suspension cultures.-Planta 220: 849-853, 2005. Go to original source...
    21. Moore, A.L., Albury, M.S., Crichton, P.G., Affourtit, C.: Function of the alternative oxidase: is it a scanvenger?-Trends Plant Sci. 7: 478-481, 2002. Go to original source...
    22. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 12: 473-497, 1962. Go to original source...
    23. Noguchi, K., Taylor, N.L., Millar, A.H., Lambers, H., Day, D.A.: Response of mitochondria to light intensity in the leaves of sun and shade species.-Plant Cell Environ. 28: 760-771, 2005. Go to original source...
    24. Pádua, M., Aubert, S., Casimiro, A., Bligny, R., Millar, A.H., Day, D.A.: Induction of alternative oxidase by excess copper in sycamore cell suspensions.-Plant Physiol. Biochem. 37: 131-137, 1999. Go to original source...
    25. Parani, M., Rudrabhatla, S., Myers, R., Weirich, H., Smith, B., Leaman, D.W., Goldman, S.L.: Microarray analysis of nitric oxide responsive transcripts in Arabidopsis.-Plant Biotechnol. J. 2: 359-366, 2004. Go to original source...
    26. Purvis, A.C., Shewfelt, R.L.: Does the alternative pathway ameliorate chilling injury in sensitive plant tissues?-Physiol. Plant. 88: 712-718, 1993. Go to original source...
    27. Rhoads, D.M., McIntosh, L.: Salicylic acid and regulation of respiration in higher plants, alternative oxidase expression.-Plant Cell 4: 1131-1139, 1992. Go to original source...
    28. Ribas-Carbo, M., Aroca, R., González-Meler, M.A., Irigoyen, J.J., Sanchez-Diaz, M.: The electron partitioning between the cytochrome and alternative respiratory pathways during chilling recovery in two cultivars of maize differing in chilling sensitivity.-Plant Physiol. 122: 199-204, 2000. Go to original source...
    29. Rychter, A.M., Mikulska, M.: The relationship between phosphate status and cyanide-resistant respiration in bean roots.-Physiol. Plant. 79: 663-667, 1990. Go to original source...
    30. Siedow, J.N., Umbach, A.L.: The mitochondrial cyanide-resistant oxidase: structural conservation amid regulatory diversity.-BBA-Bioenergetics 1459: 432-439, 2000. Go to original source...
    31. Sieger, S.M., Kristensen, B.K., Robson, C.A., Amirsadeghi, S., Eng, E.W.Y., Abdel-Mesih, A., Møller, I.M., Vanlerberghe, G.C.: The role of alternative oxidase in modulating carbon use efficiency and growth during macronutrient stress in tobacco cells.-J. exp Bot. 56: 1499-1515, 2005. Go to original source...
    32. Simons, B.H., Lambers, H.: The alternative oxidase: is it a respiratory pathway allowing a plant to cope with stress?-In: Lerner, H.R. (ed.): Plant Responses to Environmental Stresses: from Phytohormones to Genome Reorganization. Pp. 265-286. Marcel Dekker, New York 1999. Go to original source...
    33. Storey, R., Walker, R.R.: Citrus and salinity.-Sci. Hort. 78: 39-81, 1999. Go to original source...
    34. Umbach, A.L., Wiskich, J.T., Siedow, J.N.: Regulation of alternative oxidase kinetics by puruvate and intermolecular disulfide bond redox status in soybean seedling mitochondria.-FEBS Lett. 348: 181-184, 1994. Go to original source...
    35. Vandenabeele, S., Van der Kelen, K., Dat, J., Gadjev, I., Boonefaes, T., Morsa, S., Rottiers, P., Slooten, L., Van Montagu, M., Zabeau, M., Inze, D., Van Breusegem, F.: A comprehensive analysis of hydrogen peroxideinduced gene expression in tobacco.-PNAS 100: 16113-16118, 2003. Go to original source...
    36. Vanlerberghe, G.C., McIntosh, L.: Lower growth temperature increases alternative pathway capacity and alternative oxidase protein in tobacco.-Plant Physiol. 100: 115-119, 1992a. Go to original source...
    37. Vanlerberghe, G.C., McIntosh, L.: Coordinate regulation of cytochrome and alternative pathway respiration tobacco.-Plant Physiol. 100: 1846-1851, 1992b. Go to original source...
    38. Vanlerberghe, G.C., McIntosh, L.: Mitochondrial electron transport regulation of nuclear gene expression. Studies with the alternative oxidase gene of tobacco.-Plant Physiol. 105: 867-874, 1994. Go to original source...
    39. Wagner, A.M.: A role of active oxygen species as second messengers in the induction of alternative oxidase gene expression in Petunia hybrida cells.-FEBS Lett. 368: 339-342, 1995. Go to original source...
    40. Wagner, A.M., Krab, K.: The alternative respiration pathway in plants: role and regulation.-Physiol. Plant. 95: 318-325, 1995. Go to original source...
    41. Wagner, A.M., Moore, AL.: Structure and function of the plant alternative oxidase: its putative role in the oxygen defence mechanism.-Biosci. Rep. 17: 319-333, 1997. Go to original source...

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