biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:93-96, 2008 | DOI: 10.1007/s10535-008-0015-6

Callus production, somatic embryogenesis and plant regeneration of Lycium barbarum root explants

Z. Hu1,2,*, Y. Hu1, H. H. Gao2, X. Q. Guan1, D. H. Zhuang1
1 Department of Biology, Shantou University, Shantou, P.R. China
2 Institute of Cell Biology, School of Life Science, Lanzhou University, Lanzhou, P.R. China

A new micropropagation system for Lycium barbarum (L.) was developed using root explants as starting material. Callus can be produced from root explants on Murashige and Skoog (MS) medium containing 0.2 mg dm-3 2,4-dichlorophenoxyacetic acid. After three subcultures on the same medium, callus was then transferred onto the MS medium supplemented with 500 mg dm-3 lactalbumin hydrolysate to induce somatic embryogenesis (SE). After 20 d, about 60 somatic embryos per 0.25 g(f.m.) of embryogenic callus were obtained but only about 10 % of embryos converted into plantlets. After acclimated in the greenhouse, all of the randomly selected plantlets had survived and were similar phenotypically to zygotic seedlings. In addition, the effects of irradiance, photoperiod, growth regulators, explant age and cold treatment on SE of root-derived callus were evaluated.

Keywords: auxin; lactalbumin hydrolysate; medicinal plant; micropropagation
Subjects: auxins; gibberellic acid, gibberellins; in vitro culture, callus; in vitro culture, regeneration; Lycium barbarum; medicinal plants; nutrient medium, Murashige and Skoog (MS)

Received: September 1, 2005; Accepted: August 8, 2006; Published: March 1, 2008  Show citation

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Hu, Z., Hu, Y., Gao, H.H., Guan, X.Q., & Zhuang, D.H. (2008). Callus production, somatic embryogenesis and plant regeneration of Lycium barbarum root explants. Biologia plantarum, 52(1), 93-96. doi: 10.1007/s10535-008-0015-6
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    References

    1. Akashi, R., Hoffmann-Tsay, S.S., Hoffmann, F.: Selection of a super-growing legume root culture that permits controlled switching between root cloning and direct embryogenesis.-Theor. appl. Genet. 96: 758-764, 1998. Go to original source...
    2. Bekele, E., Klock, G., Zimmermann, U.: Somatic embryogenesis and plant regeneration from leaf and root explants and from seeds of Eragrostis tef (Gramineae).-Hereditas 123: 183-189, 1995 Go to original source...
    3. Bergmann, B.A., Kackett, W.P., Pellett, H.: Somatic embryogenesis in Aesculus.-In Vitro cell dev. Biol. Plant 32: 161-164, 1996.
    4. Carman, J.G.: Embryogenic cell in plant tissue cultures: occurrence and behavior.-In Vitro cell dev. Biol. Plant 26: 746-753, 1990. Go to original source...
    5. Chand, S., Sahrawat, A.K.: Somatic embryogenesis and plant regeneration from root segments of Psoralea corylifolia L., an endangered medicinally important plant.-In Vitro cell dev. Biol. Plant 38: 33-38, 2002. Go to original source...
    6. Hess, J.R., Carman, J.G.: Embryogenic competence of immature wheat embryos: genotype, donor plant environment, and endogenous hormone levels.-Crop Sci. 38: 249-253, 1998. Go to original source...
    7. Hu, Z., Ding, H.B., Wang, X., Wang, L.S.: [A comparative study on the syntheses of DNA, RNA and protein during in vitro organogenesis and somatic embryogenesis of Lycium barbarum.]-Acta Biol. exp. sin. 31: 403-411, 1998. [In Chinese.]
    8. Hu, Z., Guo, G.Q., Zhao, D.L., Li, L.H., Zheng, G.C.: Shoot regeneration from leaf explant of Lycium barbarum and Agrobalterium-mediated genetic transformation.-Russ. J. of Plant Physiol. 48: 453-458, 2001. Go to original source...
    9. Hu, Z., Wang Y.X., Wu, Y. R., Li, W.: [Effects of light and plant growth regulators on growth of normal and hairy root from Lycium barbarum in vitro.] China J. Chinese Materia med. 31: 106-110, 2006. [In Chinese.]
    10. Hu, Z., Yang, J., Guo, G.Q., Zheng, G.C.: High efficiency transfomation of Lycium barbarum and Agrobacterium tumefaciens and transgenic plant regeneration via somatic embryogenesis.-Plant Cell Rep. 21: 233-237, 2002.
    11. Jayasankar, S., Gray, D.J., Litz, R.E.: High-efficiency somatic embryogenesis and plant regeneration from suspension cultures of grapevine.-Plant Cell Rep. 18: 533-537, 1999. Go to original source...
    12. Komai, F., Okuse, I., Harada, T.: Somatic embryogenesis and plant regeneration in culture of root segments of spinach (Spinacia oleracea L).-Plant Sci. 113: 203-208, 1996. Go to original source...
    13. Luo, J.P., Jiang, S.T., Pan, L.J.: Cold-enhanced somatic embryogenesis in cell suspension cultures of Astragalus adsurgens Pall.: relationship with exogenous calcium during cold pretreatment.-Plant Growth Regul. 40: 171-177, 2003. Go to original source...
    14. Milivojeviĉ, S., Mitroviĉ, A., Ĉulafiĉ, Lj.: Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro.-Biol. Plant. 49: 35-39, 2005 Go to original source...
    15. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    16. Niu, D.S., Shou, Q.Q., Qing, J.S., Wang, L., Wang, D.Z.: [Establishment and plant regeneration of single cell culture in Lycium barbarum.]-Chinese Sci. Bull. 30: 296-298, 1985. [In Chinese.]
    17. Niu, D.S., Shou, Q.Q., Wang, L., Qing, J.S., Cheng, S.P., Cao, S.F., Wang, D.Z.: [Plant regeneration from calli of hypocotyl, shoot and young ovary of Lycium barbarum.]-Hereditas (Beijing) 5: 24-26, 1983. [In Chinese.]
    18. Ratushnyak, Y.I., Piven, N.M., Rudas, V.A.: Protoplast culture and plant regeneration in Lycium barbarum L.-Plant Cell Tissue Organ Cult. 17: 183-190, 1989. Go to original source...
    19. Ratushnyak, Y.I., Rudas, V.A., Piven, N.M.: Regeneration of Lycium barbarum L. plants from leaf tissue, callus culture and callus protoplast.-Plant Cell Rep. 9: 84-87, 1990. Go to original source...
    20. Sharma, A., Kumar, A.: Somatic embryogenic and plant regeneration from leaf-derived cell suspension of a mature tree-Thevetia peruviana L.-Plant Cell Rep. 14: 171-174, 1994. Go to original source...
    21. Wang, H.C., Chen, J.T., Wu, S.P., Lin, M.C., Chang, W.C.: Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu.-Biol. Plant. 50: 279-282, 2006. Go to original source...
    22. Wang, H.Z., Du, L.Q., Huang, F.C., Li, A.S., Shou, Q.Q.: [Agrobacterium tumefaciens-mediated transformation of Lycium barbarum and regeneration of transgenic plants.]-Sci. China (Ser. B) 23: 391-395, 1993. [In Chinese.]
    23. Yu, M.S., Yuen, W.H., So, K.F., Chang, R.C.C.: Neuroprotective effects of extracts from anti-aging Chinese medicine Lycium barbarum (Gou-Qi-Zi).-J. Neurochem. 88(Suppl.): 69-69, 2004.
    24. Zhang, M., Chen, H.X., Huang, J., Li, Z., Zhu, C.P., Zhang, S.H.: Effect of Lycium barbarum polysaccharide on human hepatoma QGY7703 cells: Inhibition of proliferation and induction of apoptosis.-Life Sci. 76: 2115-2124, 2005. Go to original source...

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