biologia plantarum

Biologia plantarum 52:97-100, 2008 | DOI: 10.1007/s10535-008-0016-5

In vitro multiplication of heavy metals hyperaccumulator Thlaspi caerulescens

J. Xu¹, Y. X. Zhang², T. Y. Chai^1,*, Z. Q. Guan¹, W. Wei¹, L. Han¹, L. Cong¹

¹ Department of Biology, Graduate University of Chinese Academy of Sciences, Beijing, China

² Department of Bioengineering, China University of Mining and Technology, Beijing, China

A micropropagation protocol through multiple shoot formation was developed for Thlaspi caerulescens L., one of the most important heavy metals hyperaccumulator plants. In vitro seed-derived young seedlings were used for the initiation of multiple shoots on Murashige and Skoog (MS) medium with combinations of benzylaminopurine (BA; 0.5-1.0 mg dm^-3), naphthaleneacetic acid (NAA; 0-0.2 mg dm^-3), gibberellic acid (GA₃; 0-1.0 mg dm^-3) and riboflavin (0-3.0 mg dm^-3). The maximum number of shoots was developed on medium containing 1.0 mg dm^-3 BA and 0.2 mg dm^-3 NAA. GA₃ (0.5 mg dm^-3) in combination with BA significantly increased shoot length. In view of shoot numbers, shoot length and further rooting rate, the best combination was 1.0 mg dm^-3 BA + 0.5 mg dm^-3 GA₃ + 1.0 mg dm^-3 riboflavin. Well-developed shoots (35-50 mm) were successfully rooted at approximately 95 % on MS medium containing 20 g dm^-3 sucrose, 8 g dm^-3 agar and 1.0 mg dm^-3 indolebutyric acid. Almost all in vitro plantlets survived when transferred to pots.

Keywords: micropropagation; growth regulators; riboflavin

Subjects: auxins; cytokinins; gibberellic acid, gibberellins; heavy metals; in vitro culture, growth control; in vitro culture, micropropagation; in vitro culture, regeneration; in vitro culture, rooting; nutrient medium, Murashige and Skoog (MS); riboflavine; Thlaspi caerulescens

Received: December 2, 2005; Accepted: August 16, 2006; Published: March 1, 2008  Show citation