biologia plantarum

Biologia plantarum 52:110-112, 2008 | DOI: 10.1007/s10535-008-0020-9

Successful micropropagation protocol of Piper methysticum

Z. Zhang^1,*, L. Zhao¹, X. Chen¹, X. Zheng¹

¹ State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, P.R. China

An efficient in vitro propagation of kava (Piper methysticum) was established. Utilizing 15-d-old tender shoots from dormant auxiliary buds as explants, significant induction of vigorous aseptic cluster shoots was achieved in Murashige and Skoog (MS) medium containing 0.5 mg dm^-3 6-benzyladenine (BA), 0.5 mg dm^-3 indole-3-acetic acid (IAA), and antibiotics after 30 d. In vitro rooting was achieved at 100 % efficiency in MS medium containing 0.75 to 1.00 mg dm^-3 IAA or indole-3-butyric acid and 3 % sucrose. The most robust and long roots were observed in medium with IBA. Moreover, the embryonic callus was induced from petioles in MS medium supplemented with 1.0 mg dm^-3 BA and 0.1 mg dm^-3 IAA, of which 70 % differentiated into shoots in the presence of 1.0 mg dm^-3 BA and 0.5 mg dm^-3 IAA.

Keywords: callus; cluster shoots; dormant auxiliary bud; endogenous contamination; growth regulators

Subjects: auxins; cytokinins; in vitro culture, callus; in vitro culture, micropropagation; in vitro culture, regeneration; nutrient medium, Murashige and Skoog (MS); Piper methysticum

Received: March 8, 2006; Accepted: December 5, 2006; Published: March 1, 2008  Show citation