biologia plantarum

Biologia plantarum 52:133-136, 2008 | DOI: 10.1007/s10535-008-0027-2

Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis

S. Gonçalves¹, H. Serra², J. M. F. Nogueira², R. Almeida¹, L. Custódio¹, A. Romano^1,*

¹ Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, Faro, Portugal

² Departamento de Química e Bioquímica e Centro de Ciências Moleculares e Materiais, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal

In this work the volatiles emitted from in vitro shoot-cultures and micropropagated plants of Lavandula viridis L'Hér. were characterized and compared with those obtained from the field-grown mother-plant, using headspace solid phase micro-extraction following by capillary gas chromatography coupled to mass spectrometry (HS-SPME-GC/MS). The headspace composition consisted mainly in oxygenated monoterpenes (66.7-79.2 %), where the major constituents emitted by the mature field-grown mother-plant, in vitro shoot-cultures and micropropagated plants were 1,8-cineole (74.0, 51.9 and 57.8 %) and camphor (2.9, 15.3 and 8.7 %), respectively. The headspace of in vitro shoot-cultures and micropropagated plants showed greater amount of α-pinene, camphene, β-pinene, β-selinene and selina-3,7(11)-diene, when compared with the field-grown mother-plant.

Keywords: capillary GC, MS; 1,8-cineole; fragrances; lavender; micropropagation; volatiles

Subjects: cytokinins; in vitro culture, growth control; in vitro culture, micropropagation; in vitro culture, regeneration; Lavandula viridis; nutrient medium, Murashige and Skoog (MS); volatiles

Received: October 25, 2006; Accepted: April 25, 2007; Published: March 1, 2008  Show citation