Biologia plantarum 52:146-148, 2008 | DOI: 10.1007/s10535-008-0030-7
Micropropagation of Ailanthus altissima and in vitro heavy metal tolerance
- 1 Istituto Biometeorologia IBIMET-CNR, Sezione di Bologna, Bologna, Italy
Ailanthus altissima, a fast-growing and contamination-resistant species is investigated for its use in areas contaminated by heavy metals. A micropropagation protocol for A. altissima was developed, cultured shoots were tested for in vitro heavy metals tolerance. Proliferation rate and shoot length were affected by 6-benzylaminopurine (BAP) and Murashige and Skoog's (MS) salt concentrations, best results were obtained in full strength MS medium supplemented with 1.32 or 2.64 µM BAP. Rooting percentage was strongly influenced by indole-3-butyric acid. Cultures of A. altissima exposed to heavy metals demonstrated a tolerance comparable to species already utilized in phytoremediation.
Keywords: shoot culture; copper; zinc; manganese; phytoremediation
Subjects: Ailanthus altissima; auxins; copper; cytokinins; heavy metals; in vitro culture, micropropagation; in vitro culture, regeneration; manganese; nutrient medium, Murashige and Skoog (MS); nutrient medium, Shenk and Hildebrandt (SH); phytoremediation; zinc
Received: November 8, 2006; Accepted: June 1, 2007; Published: March 1, 2008 Show citation
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