biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:191-194, 2008 | DOI: 10.1007/s10535-008-0043-2

Micropropagation of Harpagophytum procumbens

S. Kaliamoorthy1,*, G. Naidoo1, P. Achar1
1 School of Biological and Conservation Sciences, University of KwaZulu-Natal, Durban 0]4000, South Africa

An efficient protocol for micropropagation of Harpagophytum procumbens DC., an endangered African medicinal plant, was developed. Maximum shoot multiplication without callus was obtained from nodal explants cultured on Murashige and Skoog (MS) basal salts plus Gamborg's (B5) vitamins supplemented with 0.1 mg dm-3 indole-3-acetic acid and 5.0 mg dm-3 kinetin. The shoots were subsequently subcultured every 3 weeks on the same medium. Detached axillary shoots were transferred to MS basal salts plus B5 vitamins supplemented with various concentrations of α-naphthalene-acetic acid or indole-3-butyric acid (IBA), ranging from 0.5 to 2.5 mg dm-3 and 100 % rooting and optimal subsequent acclimatization was achieved on 1.0 mg dm-3 IBA. After 4 weeks of culture, the rooted shoots (>5 cm) were planted in pots containing peat, vermiculite and bark (2:1:1), covered with plastic domes and maintained at 25 °C for 2 weeks before being transferred to a glasshouse. Plant survival was about 40 %.

Keywords: acclimatization; auxins; cytokinins; devil's claw; grapple plant; tissue culture
Subjects: acclimatization, acclimation; auxins; cytokinins; gibberellic acid, gibberellins; grapple plant; Harpagophytum procumbens; in vitro culture, callus; in vitro culture, cell suspension; in vitro culture, micropropagation; nutrient medium, Gamborg (B5); nutrient medium, Murashige and Skoog (MS)

Received: January 12, 2007; Accepted: November 24, 2007; Published: March 1, 2008  Show citation

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Kaliamoorthy, S., Naidoo, G., & Achar, P. (2008). Micropropagation of Harpagophytum procumbens. Biologia plantarum, 52(1), 191-194. doi: 10.1007/s10535-008-0043-2
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