biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:195-199, 2008 | DOI: 10.1007/s10535-008-0044-1

Somatic embryogenesis in Araucaria angustifolia

A. L. Wendt dos Santos1, N. Steiner2, M. P. Guerra2, K. Zoglauer3, B. M. Moerschbacher1,*
1 Institute of Plant Biochemistry and Biotechnology, University of Muenster, Muenster, Germany
2 Department of Plant Science, Federal University of Santa Catarina, Florianópolis, Brazil
3 Institute of Biology, Humboldt University, Berlin, Germany

Immature and mature zygotic embryos were used as source of explants for induction of somatic embryogenesis in Araucaria angustifolia. Embryogenic cultures (EC) were only obtained from immature zygotic embryos. Basic medium, carbon source, and genotype showed a significant influence on the formation of stage I somatic embryos (SE). When EC were submitted to maturation conditions, SE continued their individual development until stage II, but mature embryos were not obtained. Proteins secreted by embryogenic cultures were, to a certain degree, genotype specific and included an extracellular class IV chitinase and β-1-3-glucanase.

Keywords: Araucariaceae; cell suspension cultures; extracellular proteins
Subjects: abscisic acid (ABA); Araucaria anguastifolia; carbon source in in vitro cultures; chitinase; cytokinins; in vitro culture, cell suspension; maltose; nutrient medium, Becwar et al. (MSG); nutrient medium, Gupta and Pullman (BM); nutrient medium, Litvay et al. (LM); nutrient medium, Murashige and Skoog (MS); proteins; sugars; zygotic embryogenesis

Received: September 13, 2005; Accepted: November 6, 2006; Published: March 1, 2008  Show citation

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dos Santos, A.L.W., Steiner, N., Guerra, M.P., Zoglauer, K., & Moerschbacher, B.M. (2008). Somatic embryogenesis in Araucaria angustifolia. Biologia plantarum, 52(1), 195-199. doi: 10.1007/s10535-008-0044-1
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