biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:222-230, 2008 | DOI: 10.1007/s10535-008-0049-9

Cloning and quantification of expression levels of two MADS-box genes from Momordica charantia

S. M. Peng1, T. Luo1, J. Y. Zhou1, B. Niu1, N. F. Lei1, L. Tang1, F. Chen1,*
1 College of Life Science, Sichuan University, Chengdu, P.R. China

MADS-box genes are known to be important for the development of flowers. Two MADS-box genes (MCAG2 and MCAG6) were isolated from the bitter gourd (Momordica charantia) female bud based on the MADS-box conserved sequences. The complete cDNA sequences of MCAG2 and MCAG6 encode a 231 and a 247 amino acid protein, respectively. Sequence comparison and phylogenetic analysis showed that MCAG2 and MCAG6 had high identities of amino acid with AG-like and AGL6-like genes, respectively. The alignment of the deduced amino acid sequence of AGL6-like genes revealed that there were two highly conserved regions in the C-terminus, which were designated AGL6 motif I and AGL6 motif II. Phylogeny reconstructions suggested that AGL6-like genes were divided into three major clades. RT-PCR analysis of the MCAG2 and MCAG6 genes showed that they were both expressed in floral organs at different levels. However, MCAG6 was also expressed highly in shoot apex. Quantitative real-time reverse transcriptase-polymerase chain reaction analysis indicated that the expression of MCAG2 was detected at high levels in carpel, whereas MCAG6 was detected at high levels in shoot apex. The gene expression patterns suggest that MCAG2 and MCAG6 have a role in regulating bitter gourd floral development.

Keywords: AGAMOUS; AGL6; bitter gourd; expression pattern; phylogeny
Subjects: amino acids; bitter gourd; Momordica charantia; polymerase chain reaction (PCR)

Received: April 10, 2006; Accepted: November 11, 2006; Published: June 1, 2008  Show citation

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Peng, S.M., Luo, T., Zhou, J.Y., Niu, B., Lei, N.F., Tang, L., & Chen, F. (2008). Cloning and quantification of expression levels of two MADS-box genes from Momordica charantia. Biologia plantarum, 52(2), 222-230. doi: 10.1007/s10535-008-0049-9
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    References

    1. Angenent, G.C., Colombo, L.: Molecular control of ovule development.-Trends Plant Sci. 1: 228-232, 1996. Go to original source...
    2. Becker, A., Theissen, G.: The major clades of MADS-box genes and their role in the development and evolution of flowering plants.-Mol. Phylogenet. Evol. 129: 464-489, 2003. Go to original source...
    3. Boss, P.K., Sensi, E., Hua, C., Davies, C., Thomas, M.R.: Cloning and characterisation of grapevine (Vitis vinifera L.) MADS box genes expressed during inflorescence and berry development.-Plant Sci. 162: 887-895, 2002. Go to original source...
    4. Bowman, J.L., Smyth, D.R., Meyerowitz, E.M.: Genetic interactions among floral homeotic genes of Arabidopsis.-Development 112: 1-20, 1991. Go to original source...
    5. Brunner, A.M., Rottmann, W.H., Sheppard, L.A., Krutovskii, K., DiFazio, S.P., Leonardi, S., Strauss, S.H.: Structure and expression of duplicate AGAMOUS orthologues in poplar.-Plant mol. Biol. 44: 619-634, 2000. Go to original source...
    6. Carlsbecker, A., Tandre, K., Johanson, U., Englund, M., Engstrom, P.: The MADS box gene DAL1 is a potential mediator of the juvenile-to-adult transition in Norway spruce (Picea abies).-Plant J. 40: 546-557, 2004. Go to original source...
    7. Cho, S., Jang, S., Chae, S., Chung, K.M., Moon, Y.H., An, G., Jang, S.K.: Analysis of the C-terminal region of Arabidopsis thaliana APETALA1 as a transcription activation domain.-Plant mol. Biol. 40: 419-429, 1999. Go to original source...
    8. Coen, E.S., Meyerowitz, E.M.: The war of the whorls, genetic interaction controlling flower development.-Nature 353: 31-37, 1991. Go to original source...
    9. Davies, B., Motte, P., Keck, E., Saedler, H., Sommer, H., Schwarz-Sommer, Z.: PLENA and FARINELLI, redundancy and regulatory interactions between two Antirrhinum MADS-box factors controlling flower development.-EMBO J. 18: 4023-4034, 1999. Go to original source...
    10. Egea-Cortines, M., Saedler, H., Sommer, H.: Ternary complex formation between the MADS-box proteins SQUAMOSA, DEFICIENS and GLOBOSA is involved in the control of floral architecture in Antirrhinum majus.-EMBO J. 18: 5370-5379, 1999. Go to original source...
    11. Favaro, R., Immink, R.G., Ferioli, V., Bernasconi, B., Byzova, M., Angenent, G.C., Kater, M., Colombo, L.: Ovule-specific MADS-box proteins have conserved protein-protein interactions in monocot and dicot plants.-Mol. Genet. Genomics 268: 152-159, 2002. Go to original source...
    12. Hecht, V., Foucher, F., Ferrandiz, C., Macknight, R., Navarro, C., Morin, J., Vardy, M.E., Ellis, N., Beltran, J.P., Rameau, C., Weller, J.L.: Conservation of Arabidopsis flowering genes in model Legumes.-Plant Physiol. 137: 1420-1434, 2005. Go to original source...
    13. Ho, W.K.K., Liu, S.C., Shaw, P.C., Yeung, H.W., Ng, T.B., Chan, W.Y.: Cloning of cDNA of α-momorcharin, a new ribosome-inactivating protein.-J. mol. Biol. 214: 625-626, 1991.
    14. Kater, M.M., Colombo, L., Franken, J., Busscher, M., Masiero, S., Van Lookeren Campagne, M.M., Angenent G.C.: Multiple AGAMOUS homologs from cucumber and petunia differ in their ability to induce reproductive organ fate.-Plant Cell. 10: 171-182, 1998. Go to original source...
    15. Kempin, S.A., Mandel, M.A., Yanofsky, M.F.: Conversion of perianth into reproductive organs by ectopic expression of the tobacco floral homeotic gene NAG1.-Plant Physiol. 103: 1041-1046, 1993. Go to original source...
    16. Kramer, E.M., Jaramillo, M.A., Di-Stilio, V.S.: Patterns of gene duplication and functional evolution during the diversification of the AGAMOUS subfamily of MADS box genes in angiosperms.-Genetics 166: 1011-1023, 2004. Go to original source...
    17. Ma, H., Yanofsky, M.F., Meyerowitz, E.M.: AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes.-Genes Dev. 5: 484-495, 1991. Go to original source...
    18. Norman, C., Runswick, M., Pollock, R., Treisman, R.: Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-Fos serum response element.-Cell 55: 989-1003, 1988. Go to original source...
    19. Passmore, S., Maine, G.T., Elble, R., Christ, C., Tye, B.K.: Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MAT-alpha cells.-J. mol. Biol. 204: 593-606, 1988. Go to original source...
    20. Pnueli, L., Abeid, M.A., Zamir, D., Nacken, W., Sommer, Z.S., Lifschitz, E.: The MADS box gene family in tomato, temporal expression during floral development.-Plant J. 1: 255-266, 1991. Go to original source...
    21. Pnueli, L., Hareven, D., Rounsley, S.D., Yanofsky, M.F., Lifschitz, E.: Isolation of the tomato AGAMOUS gene TAG1 and analysis of its homeotic role in transgenic plants.-Plant Cell 6: 163-173, 1994. Go to original source...
    22. Riechmann, J.L., Meyerowitz, E.M.: MADS domain proteins in plant development.-Biol. Chem. 378: 1079-1101, 1997.
    23. Rounsley, S.D., Ditta, G.S., Yanofsky, M.F.: Diverse roles for MADS box genes in Arabidopsis development.-Plant Cell 7: 1259-1269, 1995. Go to original source...
    24. Schwarz-Sommer, Z., Hue, I., Huijser, P., Flor, P.J., Hansen, R., Tetens, F., Lonnig, W.E., Saedler, H., Sommer, H.: Characterization of the Antirrhinum floral homeotic MADS-box gene DEFICIENS, evidence for DNA binding and autoregulation of its persistent expression throughout flower development.-EMBO J. 11: 251-263, 1992. Go to original source...
    25. Schwarz-Sommer, Z., Huijser, P., Nacken, W., Saedler, H., Sommer, H.: Genetic control of flower development by homeotic genes in Antirrhinum majus.-Science 250: 931-936, 1990. Go to original source...
    26. Shore, P., Sharrocks, A.D.: The MADS-box family of transcription factors.-Eur. J. Biochem. 229: 1-13, 1995. Go to original source...
    27. Sommer, H., Beltran, J.P., Huijser, P., Pape, H., Lonnig, W.E., Saedler, H., Schwarz-Sommer, Z.: DEFICIENS, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus, the protein shows homology to transcription factors.-EMBO J. 9: 605-613, 1990. Go to original source...
    28. Theissen, G.: Development of floral organ identity: stories from the MADS house.-Curr. Opin. Plant Biol. 4: 75-85, 2001. Go to original source...
    29. Theissen, G., Saedler, H.: MADS-box genes in plant ontogeny and phylogeny, Haeckel's 'biogenetic low' revisited.-Curr. Opin. Genet. Dev. 5: 628-639, 1995. Go to original source...
    30. Theissen, G., Saedler, H.: Floral quartets.-Nature 409: 469-471, 2001. Go to original source...
    31. Theissen, G., Strater, T., Fischer, A., Saedler, H.: Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize.-Gene 156: 155-166, 1995. Go to original source...
    32. Treves, R.P., Kahana, A., Rosenman, N., Xiang, Y., Silberstein, L.: Expression of multiple AGAMOUS-like genes in male and female flowers of cucumber (Cucumis sativus L.).-Plant Cell Physiol. 39: 701-710, 1998. Go to original source...
    33. Tsaftaris, A.S., Pasentsis, K., Polidoros, A.N.: Isolation of a differentially spliced C-type flower specific AG-like MADS-box gene from Crocus sativus and characterization of its expression.-Biol. Plant. 49: 499-504, 2005. Go to original source...
    34. Tsuchimoto, S., Van der Krol, A.R., Chua, N.H.: Ectopic expression of pMADS3 in transgenic petunia phenocopies the petunia blind mutant.-Plant Cell 5: 843-853, 1993. Go to original source...
    35. Wang, Q.M., Zeng, G.W.: Studies on expression of sex differentiation program in Momordica charantia.-J. Zhejiang Univ. (Agr. Life Sci.). 28: 243-248, 2002.
    36. Wang, S., Tang, L., Chen, F.: In vitro flowering of bitter melon.-Plant Cell Rep. 20: 393-397, 2001. Go to original source...
    37. Weigel, D., Meyerowitz, E.M.: The ABCs of floral homeotic genes.-Cell 78: 203-209, 1994. Go to original source...
    38. Yang, M.Y., Zhao, M.J., Xu, Y., Tang, L., Bai, J., Chen, F.: [Cloning and expression of a MADS box gene from bitter melon (Momordica charantia L.).]-J. Beijing Forest Univ. 26: 30-34, 2004. [In Chin.]
    39. Yanofsky, M.F., Ma, H., Bowman, J.L., Drews, G.N., Feldmann, K.A., Meyerowitz, E.M.: The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors.-Nature 346: 35-39, 1990. Go to original source...
    40. Yu, D., Kotilainen, M., Pollanen, E., Mehto, M., Elomaa, P., Helariutta, Y., Altert, V.A., Teeri, T.H.: Organ identity genes and modified patterns of flower development in Gerbera hybrida (Asteraceae).-Plant J. 17: 51-62, 1999. Go to original source...
    41. Zahn, L.M., Leebens-Mack, J.H., Arrington, J.M., Hu, Y., Landherr, L.L., dePamphilis, C.W., Becker, A., Theissen, G., Ma, H.: Conservation and divergence in the AGAMOUS subfamily of MADS-box genes, evidence of independent sub-and neofunctionalization events.-Evol. Dev. 8: 30-45, 2006. Go to original source...
    42. Zhang, J.S., Wu, Y.H., Li, Q., Li, Y.: Molecular cloning and characterization of NFBP6, a putative floral homeotic gene, from tobacco.-Sex Plant Reprod. 11: 113-116, 1998. Go to original source...

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