biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:242-250, 2008 | DOI: 10.1007/s10535-008-0053-0

Molecular cloning of CYP76A3, a novel cytochrome P450 from Petunia hybrida catalyzing the ω-hydroxylation of myristic acid

H. Imaishi1,*, U. Ishitobi1
1 Research Center for Environmental Genomics, Kobe University, Kobe, Japan

In higher plants, fatty acid hydroperoxides are intermediates in the synthesis of a diverse group of bioactive compounds. We used the reverse-transcriptase polymerase chain reaction to isolate a gene responsible for the oxidization of fatty acids from Petunia hybrida. A P450 cDNA that has not previously been isolated (CYP76A3) contained an open reading frame predicted to encode a polypeptide consisting of 507 amino acid residues. The cyp76A3 cDNA was expressed in Saccharomyces cerevisiae AH22 cells under the control of an alcohol dehydrogenase promoter and terminator. The recombinant yeast microsome containing the CYP76A3 hemoprotein was found to specifically catalyze ω-hydroxylation of myristic acid. A high level of the transcripts of the cyp76A3 gene was found in the leaves and roots of P. hybrida, but not in the stems and flowers.

Keywords: biosynthesis regulation; cytochrome monooxygenases; oxidation of fatty acids
Subjects: cytochrome oxidase; fatty acids; Petunia hybrida; photon flux density; polymerase chain reaction (PCR); Saccharomyces cerevisiae

Received: September 14, 2006; Accepted: February 21, 2007; Published: June 1, 2008  Show citation

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Imaishi, H., & Ishitobi, U. (2008). Molecular cloning of CYP76A3, a novel cytochrome P450 from Petunia hybrida catalyzing the ω-hydroxylation of myristic acid. Biologia plantarum, 52(2), 242-250. doi: 10.1007/s10535-008-0053-0
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