biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:275-280, 2008 | DOI: 10.1007/s10535-008-0058-8

Location and prokaryotic expression of low molecular mass glutanin subunit gene 177-21 from Triticum aestivum

F. Chen1,*, Z. Li1, Y. Wu1, H. Zhang1, G. Xia1
1 School of Life Science, Shandong University, Jinan, P.R. China

To characterize the low molecular mass glutenin subunit gene 177-21 (AY994364) in wheat (Triticum aestivum L. cv. Jinan 177), we developed a specific PCR primer set to decide its locus with nullisomic-tetrasomic lines of Chinese spring wheat. The result showed that it was assigned to Glu-D3. The DNA fragment of 177-21 was then subcloned into the pGEX-4T-1 expression vector and expressed in E. coli with isopropyl-1-thio-β-D-galactoside induction. The result indicated that this gene encodes about 30 kD polypeptide and deduced amino acid sequence consists of eight cysteine residues. Of the eight, six may be related with the formation of intra-molecular disulfide bonds, the last two with the formation of inter-molecular disulfide bonds, which could be a potential extender in "glutenin polymer" to have positive influence on quality of wheat flour.

Keywords: Escherichia coli; gene location; nullisomic-tetrasomic lines; prokaryotic expression
Subjects: amino acids; chromosomes; glutathione-S-transferase; plasmid; Triticum aestivum; wheat

Received: May 29, 2006; Accepted: January 17, 2007; Published: June 1, 2008  Show citation

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Chen, F., Li, Z., Wu, Y., Zhang, H., & Xia, G. (2008). Location and prokaryotic expression of low molecular mass glutanin subunit gene 177-21 from Triticum aestivum. Biologia plantarum, 52(2), 275-280. doi: 10.1007/s10535-008-0058-8
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