biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:321-328, 2008 | DOI: 10.1007/s10535-008-0066-8

Induction of pathogenesis-related proteins in sugarcane leaves and cell-cultures by a glycoprotein elicitor isolated from Colletotrichum falcatum

A. Ramesh Sundar1,*, R. Velazhahan2, S. Nagarathinam1, P. Vidhyasekaran2
1 Sugarcane Breeding Institute (ICAR), Coimbatore, India
2 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India

The induction of pathogenesis-related (PR) proteins in sugarcane (Saccharum officinarum L.) leaves and suspension-cultured cells in response to treatment with a glycoprotein elicitor isolated from Colletotrichum falcatum (the red rot pathogen) was investigated. Treatment of leaves and cells with the elicitor resulted in a much marked increase in the activities of chitinase and β-1,3-glucanase in red rot resistant (BO 91) than susceptible (CoC 671) sugarcane cultivar. SDS-PAGE analysis revealed that C. falcatum elicitor induced the accumulation of several proteins in suspension-cultured cells of resistant cultivar (BO 91); among them the 35 kDa protein was predominant. Whereas, a 27 kDa protein was induced predominantly in the cells of susceptible cultivar upon treatment with the elicitor. When sugarcane leaves were treated with C. falcatum elicitor, two proteins with apparent molecular masses of 25 and 27 kDa were induced both in the resistant and susceptible cultivars. However, the induction was stronger in the resistant than the susceptible cultivar. Immunoblot analysis for chitinase indicated that a protein with an apparent molecular mass of 37 kDa cross-reacting with barley chitinase antiserum was strongly induced in the suspension cultured cells of both the cultivars. The induction of 37 kDa chitinase was more in the cells of resistant cultivar than in the susceptible cultivar. Western blot analysis revealed that a 25 kDa thaumatin-like protein (TLP) cross-reacting with bean TLP antiserum was strongly induced in leaves and cultured cells of both resistant and susceptible cultivars due to elicitor treatment.

Keywords: chitinase; β-1,3-glucanase; red rot pathogen; Saccharum officinarum; thaumatin-like protein
Subjects: chitinase; Colletotrichum falcatum; elicitors; in vitro culture, growth control; proteins; Saccharum officinarum

Received: April 10, 2006; Accepted: December 11, 2006; Published: June 1, 2008  Show citation

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Ramesh Sundar, A., Velazhahan, R., Nagarathinam, S., & Vidhyasekaran, P. (2008). Induction of pathogenesis-related proteins in sugarcane leaves and cell-cultures by a glycoprotein elicitor isolated from Colletotrichum falcatum. Biologia plantarum, 52(2), 321-328. doi: 10.1007/s10535-008-0066-8
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