biologia plantarum

Biologia plantarum 52:339-342, 2008 | DOI: 10.1007/s10535-008-0069-5

In vitro regeneration of medicinal plant Centella asiatica

H. Mohapatra¹, D. P. Barik¹, S. P. Rath^1,*

¹ Post Graduate Department of Botany, Utkal University, Vanivihar, Bhubaneswar, Orissa, India

This paper describes multiple shoot regeneration from leaf and nodal segments of a medicinally important herb Centella asiatica L. on Murashige and Skoog's (MS) medium supplemented with a range of growth regulators. The highest number of multiple shoots was observed on MS augmented with 3.0 mg dm^-3 N⁶-benzylaminopurine (BAP) and 0.05 mg dm^-3 α-naphthaleneacetic acid (NAA). Leaf explant showed maximum percentage of cultures regenerating shoots (81.6 %), with the highest shoot number (8.3 shoots per explant) and the shoot length (2.1 cm) whereas, nodal explant showed less number of shoots with callus formation at the base cut end. Successive shoot cultures were established by repeatedly sub-culturing the original explant on a fresh medium. Rooting of in vitro raised shoots was best induced on half strength MS supplemented with 0.5 mg dm^-3 indole-3-butyric acid (IBA) with highest percentage of shoot regenerating roots (76.8 %) with 3-4 roots per shoot. Plantlets were acclimated in Vermi-compost and eventually established in soil. Contents of chlorophyll, total sugars, reducing sugars and proteins were estimated in leaf tissue from both in vivo and in vitro raised plants. Chlorophyll content was higher in in vivo plants, whereas other three components were higher in in vitro plants.

Keywords: biochemical parameters; direct regeneration; leaf explant; growth regulators

Subjects: auxins; Centella asiatica; chlorophyll a,b; in vitro culture, regeneration; medicinal plants; nutrient medium, Gamborg (B5); nutrient medium, Murashige and Skoog (MS); proteins; sugars

Received: January 11, 2006; Accepted: November 11, 2006; Published: June 1, 2008  Show citation