biologia plantarum

Biologia plantarum 52:343-346, 2008 | DOI: 10.1007/s10535-008-0070-z

A novel cadmium induced protein in wheat: characterization and localization in root tissue

B. Mittra^1,*, S. Sharma², A. B. Das³, S. L. Henry⁴, T. K. Das⁵, P. Ghosh⁶, S. Ghosh⁶, P. Mohanty^3,7

¹ Department of Biosciences and Biotechnology, Fakir Mohan University, Vayasa Vihar, Balasore, Orissa, India

² Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India

³ Cytogenetics Laboratory, Regional Plant Resource Center, Nayapalli, Bhubaneswar, Orissa, India

⁴ Department of Plant Sciences, Institute of Bio-medical and Life Sciences, University of Glasgow, UK

⁵ Electron Microscopic Facility, All India Institute of Medical Sciences, New Delhi, India

⁶ Department of Biophysics, University of Delhi, South Campus, New Delhi, India

⁷ School of LifeSciences, University of Hyderabad, Hyderabad, Andhra Pradesh, India

A 51-kDa soluble protein was over-expressed in wheat (Triticum aestivum) seedlings by the treatment of seeds before germination with 50 µM CdCl₂ for 48 h and subsequently washed off Cd²⁺. This protein designated as Cd stress associated protein (CSAP), was purified. Polyclonal antibody was raised against CSAP for localizing the protein in root tissue of treated and control seedlings. It was observed that CSAP was located below the plasma membrane and outer periphery of the tonoplast. This unique type of organized localization of CSAP is suggestive of defensive role against metal phytotoxicity. N-terminal analysis of CSAP and expressed sequence tags (EST) database search of wheat sequences suggests that this protein has not been reported earlier in higher plants.

Keywords: cadmium stress; immunolocalization; N-terminal sequencing; stress protein; Triticum aestivum

Subjects: heavy metals; immunolocalization; in vitro culture, rooting; proteins; Triticum aestivum; wheat

Received: May 9, 2006; Accepted: January 5, 2007; Published: June 1, 2008  Show citation