biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:377-380, 2008 | DOI: 10.1007/s10535-008-0079-3

Syringin production by Saussurea medusa cell cultures in a novel bioreactor

C. M. Xu1,2, Y. Ou2, B. Zhao2, X. D. Wang2, X. F. Yuan2, Y. C. Wang2
1 Beijing Key Laboratory of Plant Resources Research and Development, College of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing, P.R. China
2 Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China

The culture of Saussurea medusa cell were cultured in an internal loop airlift bioreactor with sifter draft tube (ILABSDT) was investigated. Under the optimal culture conditions, which were inoculation size 1.5 g(d.m.) dm-3, aeration rate 0.3 dm3(air) dm-3(medium) min-1, and 14 mesh sifter holes, the maximum biomass, syringin content and syringin production reached 11.7 g(d.m.) dm-3, 17.7 mg g-1 and 206.6 mg dm-3, respectively. Among cell cultures in shake flask, bubble column bioreactor and ILABSDT, ILABSDT had the highest syringin productivity and reached 12.41 mg dm-3 d-1.

Keywords: aeration rate; inoculation size; mesh; sifter draft tube
Subjects: bioreactor; in vitro culture, growth control; Saussurea medusa

Received: September 11, 2006; Accepted: March 22, 2007; Published: June 1, 2008  Show citation

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Xu, C.M., Ou, Y., Zhao, B., Wang, X.D., Yuan, X.F., & Wang, Y.C. (2008). Syringin production by Saussurea medusa cell cultures in a novel bioreactor. Biologia plantarum, 52(2), 377-380. doi: 10.1007/s10535-008-0079-3
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