biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:475-485, 2008 | DOI: 10.1007/s10535-008-0093-5

Gene expression profiling in maize roots under aluminum stress

G. M. A. Cançado1,*, F. T. S. Nogueira1, S. R. Camargo1, R. D. Drummond1, R. A. Jorge2, M. Menossi1
1 Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, Brazil
2 Instituto de Química, Universidade Estadual de Campinas, Campinas, Brazil

To investigate the molecular mechanisms of Al toxicity, cross-species cDNA array approach was employed to identify expressed sequence tags (ESTs) regulated by Al stress in root tips of Al-tolerant maize (Zea mays) genotype Cat100-6 and Al-sensitive genotype S1587-17. Due to the high degree of conservation observed between sugarcane and maize, we have analyzed the expression profiling of maize genes using 2 304 sugarcane (ESTs) obtained from different libraries. We have identified 85 ESTs in Al stressed maize root tips with significantly altered expression. Among the up-regulated ESTs, we have found genes encoding previously identified proteins induced by Al stress, such as phenyl ammonia-lyase, chitinase, Bowman-Birk proteinase inhibitor, and wali7. In addition, several novel genes up-and downregulated by Al stress were identified in both genotypes.

Keywords: abiotic-stress; Al-sensitive; Al-tolerant; heterologous-hybridization; Saccharum sp; Zea mays
Subjects: cultivar and genotype differences; heavy metals; maize; Saccharum sp.; Zea mays

Received: December 15, 2006; Accepted: May 24, 2007; Published: September 1, 2008  Show citation

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Cançado, G.M.A., Nogueira, F.T.S., Camargo, S.R., Drummond, R.D., Jorge, R.A., & Menossi, M. (2008). Gene expression profiling in maize roots under aluminum stress. Biologia plantarum, 52(3), 475-485. doi: 10.1007/s10535-008-0093-5
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