biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:507-512, 2008 | DOI: 10.1007/s10535-008-0098-0

Nucleolar vacuolation in soybean root meristematic cells during recovery after chilling

D. Stępiński1,*
1 Department of Cytophysiology, University of ŁódĽ, ŁódĽ, Poland

The nucleolar vacuole formation in soybean root meristematic cells from seedlings grown 3 d at temperature 25 °C (control), 3 d at temperature 25 °C and then transferred to 10 °C (chilling) for 4 d, and after recovery for 1.5, 3, 6, 12 and 24 h at 25 °C were observed on semi-thin sections. Simultaneously, autoradiographic studies with 3H-uridine on squashed preparations were carried out. During recovery of plants, the number of vacuolated nucleoli increased gradually from 24 % after 1.5 h up to 40 % after 24 h, while in the control there were 18 % of nucleoli with vacuoles and after 4-d chilling only 5 %. Labelling of cells during 20-min incubation in 3H-uridine and during 80-min post-incubation in non-radioactive medium was increased in recovered plants in comparison with the control and chilled plants. The conclusion has been drawn that nucleolar vacuoles in soybean plants are formed as a result of migration of granular component accumulated in nucleolus during 4-d chilling.

Keywords: Glycine max; 3H-uridine incorporation
Subjects: Glycine max; in vitro culture, rooting; photon flux density; soybean; vacuole

Received: April 7, 2006; Accepted: May 4, 2007; Published: September 1, 2008  Show citation

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Stępiński, D. (2008). Nucleolar vacuolation in soybean root meristematic cells during recovery after chilling. Biologia plantarum, 52(3), 507-512. doi: 10.1007/s10535-008-0098-0
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